Density Gradient Medium From Corning That Performs Like Ficoll

George Washington University

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Company:

Corning

Product Name:

Lymphocyte separation medium

Catalog Number:

25-072-CV

The goal of this research is to isolate peripheral blood mononuclear cells, then separate CD14+ cells, and use them to study dendritic cell uptake mechanisms.

Experimental Design and Results Summary

Application

PBMC isolation

Starting Material

Leukopak blood sample

Tips

Use as you would use Ficoll or other density gradient media.

Results Summary

This product helped us isolate a buffy coat containing PBMCs without red blood cell or granulocyte infiltration. Our viability and downstream applications have been reliable using this medium.

DOI or PMID #

N/A

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Summary

The Good

Great layers and might have lower toxicity than ficoll.

The Bad

No drawback, works great!

The Bottom Line

If you're looking for a Ficoll alternative, this one will work great.

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