HMGB1 From BioLegend Gave Me Satisfactory Results When Used for Rat Samples

November 08, 2023

BlueskyReddit
Biotech
CNC
Research Scientist

Overall

Quality of Results

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Company:

Abcam

Product Name:

Rabbit Anti-HMGB1 antibody

Catalog Number:

ab18256

Clone Number:

This antibody was used to detect HMGB1 levels in rat samples (formalin-fixed, paraffin-embedded). Extravasation of HMGB1 from the nuclei has been observed in the context of aging.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Rat

Primary Incubation

1:250

Blocking Agent

Normal Goat Serum diluted in 0.1% BSA

Secondary Incubation

Alexa Fluor 555 goat anti-rabbit

Tertiary Incubation

None

Detection

Confocal - fluorescence

Results Summary

Using this antibody to detect HMGB1 levels provides a great signal with a low background. In the image it is possible to distinguish cells positive and negative for HMGB1.Image: HMGB1 (red), DAPI (nuclei)

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Usually works well in different tissues/samples.

The Bad

None.

The Bottom Line

Great antibody for rat samples. Use Tris-EDTA as antigen retrieval.

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