Excellent and Easy to Use Compensation Beads From BioLegend

November 09, 2023

BlueskyReddit
Nottingham Trent University
JvGCRC
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Compensation Beads

Catalog Number:

424602

Supplied ready to use, this simple compensation bead kit contains positive and negative beads that work with a wide range of species antibodies. Have been used on traditional and spectral flow cytometers and have worked well on both.

Experimental Design and Results Summary

Application

Flow cytometry

Starting Material

Fluorescently conjugated primary antibodies

Tips

Best results obtained when excess antibody was washed off after incubation.

Results Summary

Easy to use kit that works exactly as described. Very useful for the more unusual species as these work with rabbit, donkey, hamster and human antibodies as well as rat and mouse. Binding to positive beads was clear and well separated from negative.

DOI or PMID #

N/A

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Summary

The Good

Can be used with antibodies raised in a range of species (inc donkey and armenian hamster).

The Bad

It is required to wash off excess antibody before running.

The Bottom Line

Easy to use and provide reliable results for antibodies raised in many different species.

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