Myc Tag (9B11) Mouse mAb From Cell Signaling Works Well for Protein Detection

UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technologies

Product Name:

Myc Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate)

Catalog Number:

2233S

Clone Number:

Antibody was used for staining tagged membrane proteins for FAC isolation and for expression level profiling by flow cytometry. The product was very good in both applications.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Human T cells

Primary Incubation

1:100, 4 degrees C, 30 minutes

Blocking Agent

2% FBS in PBS

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

Used to determine presence of tagged membrane proteins and for assaying changes in expression level by flow cytometry, Very strong signal with no background signal detected.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Strong signal and high specificity.

The Bad

None.

The Bottom Line

Worth the purchase. One of the most reliable antibodies I have used.

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