Good Flow Cytometry Marker for Live /Dead Staining From Tonbo Biosciences

PRIMATE RESEARCH CENTER
UNIVERSITY OF WISCONSIN
Scientist I

Overall

Quality of Results

Ease-of-Optimization

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Company:

Tonbo Biosciences

Product Name:

Ghost Violet 540 viablitiy dye

Catalog Number:

130-0879-T100

Clone Number:

Ghost Dye Violet 540 is a viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. The dye is excited by the violet laser and can easily distinguish between live cells and dead/necrotic cells.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Human/NHP ESC/iPSC derived cells

Primary Incubation

0.5ul in 100ul of FACS buffer containing 1e6 cells

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

The viability dye is a easy to use dye that can very nicely distinguish between viable and non viable cells in flow cytometry.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very easy to use.

The Bad

None

The Bottom Line

This viability dye could be useful for gating out non viable cells from viable cells in flow cytometry.

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