Slightly Dim, but Solid Anti-Mouse CD8a Antibody From BioLegend

WCM
Postdoctoral Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 711™ anti-mouse CD8a Antibody

Catalog Number:

100747

Clone Number:

This anti-CD8a mouse antibody provides staining on LPMCs but is a bit dim at 1:200 dilution and smears slightly.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

LPMC

Primary Incubation

1:200 in FACS buffer, 20min

Blocking Agent

Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

None

Results Summary

This anti-CD8a mouse antibody provides staining on LPMCs, but is a bit dim at 1:200 dilution and smears a slightly. Use at 1:100 for a better distinction.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Convenient fluorochrome.

The Bad

A bit dim and no separation of populations.

The Bottom Line

Solid antibody if needed in this fluorochrome, but does not provide good distinction of positive population.

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