Myc Tag (9B11) Mouse mAb From Cell Signaling Works Great

UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technologies

Product Name:

Myc Tag (9B11) Mouse mAb (PE Conjugate)

Catalog Number:

3739S

Clone Number:

N/A

The antibody has an excellent signal with low background staining. Highly specific for membrane-expressed tag staining.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Human T cells

Primary Incubation

1:100, 4 degrees C, 30 minutes.

Blocking Agent

2% FBS in PBS

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

Expression levels of tagged membrane protein expression introduced by lentivirus in mammalian cells. I was able to determine both presence and different expression levels clearly by flow cytometry.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Highly specific and strong signal.

The Bad

None.

The Bottom Line

Worth buying.

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