Thermo Fisher Scientific
Alpha-Smooth Muscle Actin Monoclonal Antibody
MA1-06110
N/A
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Our research focuses on understanding multiple pathological processes in ocular diseases. The specific goal of this experiment was to evaluate the effects of age on myofibroblast transformation. This antibody provided excellent staining for smooth muscle actin positive myofibroblasts.
Immunofluorescence
Cultured mouse fibroblast
1:500 at 4 degrees celsius for overnight
5% normal donkey serum
Alexa fluor anti-mouse IgG at 1:500 for 1 hr at room temp
Fluorescence
Immunofluorescence labeling with this antibody provided robust fluorescence signals specific to smooth muscle actin expressed in myofibroblasts (red).
Robust signals.
None.
This antibody is highly recommended for detecting fibroblast to myofibroblast transformation in vitro.