CD45 Antibody From BioLegend Gives Clear Staining

November 06, 2023

BlueskyReddit
Medicine
Weill Cornell Medicine
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 605™ anti-mouse CD45 Antibody

Catalog Number:

103140

Clone Number:

30-F11

When used for flow cytometry on intestinal cells, there will be some background, but the hematopoietic population is clear.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse caecum cells

Primary Incubation

1/200

Blocking Agent

FBS

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fortessa II

Results Summary

Mouse intestinal immune cells stained with this antibody show a clear population of CD45+ cells. I rate the staining as good but comparable to other validated antibodies I used targeting this marker.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Clear CD45 positive population.

The Bad

Some background is present.

The Bottom Line

The fluorochrome of this antibody will lead to some compensation issues, but overall staining is decent and background can be decreased with proper Titration.

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