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Alexa Fluor® 647 anti-human CD25 Antibody
302618
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The antibody works well enough for the detection of the protein, but there is a noticeable level of background staining present. This does not detract from the quality of the data significantly.
Flow cytometry
Human T cells
1:100, 4 degrees C, 30 minutes
2% FBS in PBS
None
Used to measure levels of CD25 following activation of the T cell signaling pathway. Easily able to identify cells that were high in CD25, but results seem digital in my hands.
N/A
Strong signal.
Moderate level of background signal.
Decent antibody that does its job. High signal comes at the cost of background.