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Exceptional DNA Ladder From NEB
(1)
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New England Biolabs
November 02, 2023
I use this DNA molecular marker daily. It has great resolution using a small amount. I use only 3 uL per gel and the bands always look very ...
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One of the Best Protease Inhibitors Out There From Millipore Sigma
(1)
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MilliporeSigma
November 02, 2023
cOmplete, Mini, EDTA-free Protease Inhibitor Tablets, inhibit a broad spectrum of serine and cysteine proteases. In contrast to other ...
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PNA Probe (Cy3) for FISH Staining From Panagene
(1)
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Panagene, Inc.
November 02, 2023
PNA probe (Cy3) was used for the detection of telomeres in rat samples (PBMCs, heart). To this end, an immune-FISH protocol was followed.
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Decent Anti-CD69 Antibody From BioLegend for Flow Cytometry
(1)
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BioLegend
November 01, 2023
The BV785 CD69 antibody can be used to identify recently activated CD8+ T cells within immune cell populations isolated from mouse lungs. I ...
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Fixation Buffer of Superior Quality From BD Biosciences
(1)
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November 01, 2023
My goal was to examine dendritic cell populations to determine whether certain treatments resulted in more DC activation than others.
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Mouse CD45 Antibody From BioLegend Has Great Binding Capacity
(1)
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BioLegend
November 01, 2023
Lamina propria cells from the small intestine were stained to analyze different immune cells in the lymphocyte population. The cells were ...
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Excellent Cell Culture Flask From VWR With Tightly Sealed Caps
(1)
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VWR Scientific Products
November 01, 2023
Great for cell culture. The holes provide gas exchange without allowing contamination. It has a good seal and the tissue culture bottom ...
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A Nice Ladder From Thermo Fisher to Measure DNA Fragments From 50 to 1 kB Base Pairs
(1)
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Thermo Fisher Scientific
November 01, 2023
The study aimed to identify the products of DNA restriction digestion, in agarose gel, which yields DNA fragments of 100 to 250 base pairs. ...
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Bright and Reliable Secondary Antibody From Thermo Fisher
(1)
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Thermo Fisher Scientific
November 01, 2023
The goal of this experiment was to titrate the correct amount of peptide and incubation time needed for the MHC presentation of the peptide ...
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Western Blot Membrane Stripping Reagent From ThermoFisher
(1)
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Thermo Fisher Scientific
October 31, 2023
Western blotting stands out as a highly effective method for comparing protein expression levels, offering exceptional sensitivity. However,...
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Protein Ladder From GenDepot With Two Different Size Indicators
(1)
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October 31, 2023
The use of a protein ladder is crucial for accurately determining protein sizes through SDS-PAGE analysis. In my experiments, I have relied ...
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Excellent APC/Fire™ 750 Anti-Human CD8a Antibody From BioLegend
(1)
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BioLegend
October 31, 2023
This antibody has been used to optimize flow cytometry-based immunophenotyping of PBMC samples. The results have not yet been published. ...
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Good Spark Blue™ 550 Anti-Human CD4 Antibody From Biolegend
(1)
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BioLegend
October 31, 2023
This antibody has been used to optimize flow cytometry-based immunophenotyping of PBMC samples. The results have not yet been published. ...
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Great for Sonicating Nanoparticles
(1)
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October 25, 2023
We use this instrument to sonicate our nanoparticles. This machine is easy to clean and perfectly does the job.
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Superb Antifungal Reagent for Media From MilliporeSigma
(1)
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MilliporeSigma
October 24, 2023
Amphotericin B is a polyene antifungal antibiotic from Streptomyces sp. that is clinically used to treat systemic fungal infections. It has ...
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Anti-Mouse CD8 Antibody From Biolegend With Decent Staining
(1)
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BioLegend
October 24, 2023
This antibody provides good staining quality and specificity. The background is minimal and can be improved with titration.
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BioLegend's Anti-Mouse CD4 BV421 Works Fine
(1)
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BioLegend
October 24, 2023
This antibody provides a good CD4 mouse staining with a Log3 separation and acceptable background. Background can be reduced and staining ...
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Spark Violet™ 538 Anti-Human CD3 Antibody From Biolegend Works Well
(1)
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BioLegend
October 24, 2023
This antibody has been used to optimize flow cytometry-based immunophenotyping of PBMC samples. The results have not yet been published. ...
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Solid Staining With Brilliant Violet 785™ Anti-Mouse CD45 Antibody From BioLegend
(1)
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BioLegend
October 24, 2023
Bright CD45 stain in convenient fluorochrome. Good separation of hematopoietic vs non-hematopoietic cells.
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Good Staining for GATA3 With Antibody From BD Biosciences
(1)
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October 24, 2023
Gata3 staining with this antibody is good. Separation of the positive population could be a bit better, but staining in T cells is difficult...
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Average Phospho-Histone H2A.X From Cell Signaling
(1)
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Cell Signaling Technology
October 24, 2023
Phospho-Histone H2A.X (H2A histone proteins) antibody was used in rat peripheral blood mononuclear cells (PBMCs) samples.
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Solid Dye From Cytek Biosciences for Live/Dead Cell Staining
(1)
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Tonbo Biosciences
October 23, 2023
This live/dead stain delivers solid results on mouse and human LPMCs. Optimal staining result at 1:1000.
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Selected Stable Gene Expression in HEK293 Cells With Disulfate Salt Solution From Merck
(1)
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MilliporeSigma
October 23, 2023
G418 stock was diluted to 250 mcg/ml in DMEM containing 10% FBS, 4 mM glutamine, 10 mM HEPES and puromycin (5 mcg/ml) and used to culture ...
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Prominent Antibody From BioLegend
(1)
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BioLegend
October 23, 2023
We are an immunotherapy-focused group interested in CD8 T-cells and their TCRs. We routinely use T-cell-based assays for our experimental ...
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Vacuum Driven Filtration System From Millipore is Great for Filtering Small Cells
(1)
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MilliporeSigma
October 23, 2023
We use this for filtering small amounts of reagent or cell media. Works quickly and easily and helps prevent contamination.
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Leak Proof Cell Culture Flask From Greiner
(1)
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Greiner Bio-One GmbH
October 23, 2023
Great for cell culture. The holes provide gas exchange without allowing contamination. Has a good seal and the tissue culture bottom allows ...
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Multi Use PCR System From Thermo Fisher
(1)
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Thermo Fisher Scientific
October 23, 2023
The StepOnePlus Real-Time PCR System is a highly versatile platform that excels in accommodating a wide range of PCR applications. Its ...
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Bright and Specific Anti-CD103 Antibody From BioLegend for Flow Cytometry
(1)
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BioLegend
October 23, 2023
The PEdazzle594 CD103 antibody can be used to identify tissue resident CD8+ T cells within immune cell populations isolated from mouse lungs...
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Decent Antibody From Thermo Fisher but, Not the Best
(1)
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Thermo Fisher Scientific
October 20, 2023
When analyzed by flow cytometry, there is some variability in the staining, which produces a smear. Decent, but not the best.
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Great Kit From Qiagen for Bacterial Plasmid DNA Extraction
(1)
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October 20, 2023
We use this kit daily in my lab. Simple and fast, it yields high-quality DNA for any downstream applications. After the transformation of ...
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Good Anti-KLRG1 Antibody From BioLegend for the Flow Cytometric Detection of Effector T Cells
(1)
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BioLegend
October 20, 2023
The BV 711 KLRG1 antibody can be used for identifying effector CD8+ T cells within immune cell populations isolated from mouse lungs, spleen...
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Puromycin From Thermo Fisher is Ideal for Antibiotic Selection
(1)
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Thermo Fisher Scientific
October 20, 2023
Puromycin (puromycine) is an aminoglycoside antibiotic originating from Streptomyces alboniger. It acts as an aminoacyl tRNA analog and ...
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Foolproof Nuclear Stain From Thermo Fisher
(1)
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Thermo Fisher Scientific
October 20, 2023
We do immunofluorescence experiments to visualize cells seeded on chips. This nuclear stain has never failed us in visualizing cell nuclei.
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One of the Best Nuclease Free Waters From Thermo Fisher
(1)
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Thermo Fisher Scientific
October 20, 2023
The aim of the study was real-time quantification of a gene of interest in human PBMCs. The qRT-PCR reagent was prepared with nuclease-free ...
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Cdkn1a Probe From ACD Bio for RNA-ISH in Rat Samples
(1)
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ACD Systems International Inc.
October 20, 2023
We used p21 probe (Rn-Cdkn1a) to perform RNA-ISH in rat samples. Probe with ref. 42851 from ACD is great for detecting mRNA levels of cdkn1a...
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High Quality Rheometer From Anton Paar for Measuring Pluronics
(1)
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October 19, 2023
The Modular Compact Rheometer MCR302e is an exceptional rheometer with versatile capabilities, making it an ideal instrument for measuring ...
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A Great IBA1 Antibody From Fujifilm for Immunohistochemistry
(1)
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October 19, 2023
I've used this antibody to study the localization and morphology of microglia/macrophages in a mouse retina. Immunostaining of a cross-...
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Reliable Human T3 ELISA Kit From Boster
(1)
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BosterBio
October 19, 2023
This experiment was used to quantify the levels of T3 in humans from dried blood spots for the use of at-home test kits.
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Rapid Glutamine Quantification Kit From BioVision
(1)
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October 19, 2023
I have used this glutamine colorimetric assay kit to understand the metabolic reprogramming in virus-infected primary human epithelial cells...
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Filter Plate From Millipore Suitable for ELISPOT or MTS Assays
(1)
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MilliporeSigma
October 19, 2023
We use these plates to coat INF-y antibodies for ELISPOT or for cytotoxic assays. It is sturdy with deep wells for easy multi-channel ...
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Extraordinary Antibiotic From Thermo Fisher
(1)
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October 18, 2023
Blasticidin S is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It is a potent inhibitor of protein ...
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Superb Reagent to Re-stimulate Human T-Cells
(1)
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Miltenyi Biotec
October 18, 2023
I used cytoStim reagent as a positive control to restimulate CD4+ and CD8+ T-cells present in PBMCs. After stimulation for 4-8h PBMCs were ...
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Cdkn2a Probe From ACD for the Accurate Detection of mRNA Levels
(1)
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October 18, 2023
We used p16 probe (Rn-Cdkn2a) to perform RNA-ISH in rat samples. Probe with ref. 423861 from ACD is great for detecting mRNA levels of ...
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Excellent Dye From Invitrogen for Nuclear Counterstain (DAPI)
(1)
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Thermo Fisher Scientific
October 18, 2023
DAPI is a water-soluble dye and emits blue fluorescence upon binding to AT regions of DNA which makes it a popular nuclear counterstain. ...
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Great Kit for Live/Dead Flow Calibration
(1)
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Thermo Fisher Scientific
October 18, 2023
We use these beads for live/dead compensation tubes. Works well with zombie green dye. We use one drop of reactive beads first, then add the...
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Reagent From Gibco Works Very Well to Dislodge Cells While Being Gentler Than Trypsin
(1)
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Thermo Fisher Scientific
October 17, 2023
Works very well to dislodge cells while being gentler than trypsin so we can still perform flow cytometry on them
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Satisfactory PCR Tubes From Applied Biosystems™
(1)
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October 17, 2023
MicroAmp Fast Reaction Tubes are highly durable and chemically resistant, making them ideal for PCR applications, as they can withstand ...
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DHEA Competitive ELISA Kit From Boster
(1)
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BosterBio
October 17, 2023
We used this kit to analyze saliva DHEA levels, with great success level. Validation of this assay has been straightforward. The assay ...
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Superb Kit From BioLegend for Live/Dead Cell Analysis
(1)
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BioLegend
October 17, 2023
Does a great job staining dead cells for accurate live/dead analysis in flow cytometry. Be sure to keep it in the dark, as it is light-...
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Antibody From StressMarq for Detecting P. falciparum HSP70 in Clinical Samples is Up to Standard
(1)
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StressMarq Biosciences Inc
October 17, 2023
We aimed to determine the level of pfHSP70, one of the Plasmodium falciparum chaperone proteins, in the peripheral blood of malaria patients...
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