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Date Published
Reliable and Reproducible Results with CD11b Antibody
(1)
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Miltenyi Biotec
April 07, 2023
We use CD11b in our panel within a flow cytometry experiment to identify specific antigens on the cells as a marker of inflammation. Our ...
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Easy and Quick Way to Select Stable Cells after Transfection
(1)
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Thermo Fisher Scientific
April 07, 2023
We do use in our lab Puromycin as a selection antibiotic agent in mammalian cell culture systems at a concentration of 0.5 µg/mL. After ...
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CD13 - A Bright Marker for Myeloid Cells
(1)
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April 07, 2023
Immunophenotyping of human hematolymphoid cells requires the combination of several antibodies to help identify the different white blood ...
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Good PCR Master Mix for Blood Samples
(1)
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New England Biolabs
April 07, 2023
Use this PCR master mix to amplify DNA fragment from dried blood spot. It is easy to use and no need to purify the genomic DNA and can get ...
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Reliable Methylglyoxal Antibody from Novus Biologicals
(1)
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Novus Biologicals, a Bio-Techne Brand
April 06, 2023
This antibody worked well at a 1:100 dilution. I included a picture with an FMO and methylglyoxal treated cells as a positive control.
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Great Resolution with PE Anti-Mouse Ki-67 Antibody
(1)
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BioLegend
April 06, 2023
To titrate the antibody, 2 x 10^6 splenocytes from C57BL/6 mouse were stained with: PerCP-Cy5.5 anti-CD4 Ab and Zombie NIR in 100 µL PBS at ...
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Multichannel Pipette 200uL by ClearLine
(1)
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Dutscher Scientific UK Ltd
April 06, 2023
I used this equipment for cell culture when I have to use 96-well plates. I am working every day in cell culture so this is a must-have ...
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Easy-to-Use Stripping Buffer from Serva
(1)
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SERVA
April 05, 2023
The Serva BlueClear SB is a easy-to-handle mild stripping buffer which we regularly use in our lab to strip antibodies off Western Blot ...
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Fast, High Yield Exosome/EV Isolation
(1)
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April 05, 2023
The miRCURY exosome kit was used to isolate EVs from conditioned media of 2D stem cell and cardiac cell cultures. Pelleted EVs were analyzed...
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Sensitive and Accurate Caliper to Measure Skin Thickness
(1)
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Mitutoyo
April 05, 2023
The nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the ...
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Great, Reliable Mini Prep Kit from Zymo Research
(1)
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April 05, 2023
We are interested in studying the mechanisms responsible for ensuring T cell self tolerance that rely on thyme and extra-thymic Aire ...
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Great Resolution with BioLegend's BV650 Anti-Mouse CD11c Antibody
(1)
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BioLegend
April 05, 2023
To titrate the antibody, 2 x 10^6 splenocytes from C57BL/6 mouse were stained with: PerCP-Cy5.5 anti-CD4 Ab and BV421 anti-CXCR6 Ab in the ...
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Good Tail Lysis Buffer from Viagen Biotech
(1)
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Viagen
April 05, 2023
We study the CNS basement membrane and blood-brain barrier. Specifically the role of different CNS cell types in BBB maintenance.
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Good Ultrasonic Cleanser
(1)
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Branson Ultrasonics Corp.
April 04, 2023
We study the CNS basement membrane and blood-brain barrier. Specifically the role of different CNS cell types in BBB maintenance.
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Great Ghost Dye™ from Tonbo Bioscience
(1)
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Tonbo Biosciences
April 04, 2023
I have been using this Ghost Dye™ Violet 450 to stain viable and non-viable cells in flow cytometry assays in several projects. This dye is...
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Very Good Budget Plasmid Mini-Prep Kit
(1)
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April 04, 2023
We use the kit for isolating plasmid DNA from mini cultures. It has a very good yield and purity, only downside is 10 minutes centrifuge ...
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Great Signal with Glut4 (IF8) Antibody
(1)
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Santa Cruz Biotechnology, Inc.
April 04, 2023
I worked on different cell lines stimulated with TGF-b. I always was able to detect the bands. Due the stimulation there is an increasing of...
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Very Sensitive ELISA Kit for Evaluation of Cytotoxicity
(1)
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BioLegend
April 04, 2023
ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation. I could be used for ...
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NRF1 Detection in Western Blot
(1)
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Abcam
April 04, 2023
NRF1 protein in human mesenchymal stem cells (hMSCs) was detected using an antibody-based approach. To prepare the antibody, it was diluted ...
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Reliable cDNA Synthesis Kit from Applied Biosystems
(1)
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April 03, 2023
The nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the ...
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Reliable TNFRSF11B (OPG) Human ELISA Kit
(1)
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Thermo Fisher Scientific
April 03, 2023
We used this ELISA kit several times. The kit is pretty sensitive, and has different control for serum samples or cell samples.
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Efficient Recombinant Human IFN-Gamma from BioLegend
(1)
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BioLegend
April 03, 2023
This is a recombinant Human IFN-Y, which I used for activation of human monocyte cell line. THP-1 monocytes are differentiated into ...
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Easy to Use Anti-CD3 Antibody from Thermo Fisher Scientific
(1)
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Thermo Fisher Scientific
April 03, 2023
Tumors were harvested and stained with 1 µg of CD3 Monoclonal Antibody, Brilliant Ultra Violet 737 (BUV737) (right). Cells in the Cd45+ gate...
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Very Good GFP Antibody
(1)
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April 03, 2023
We are using this antibody for HEK293 and COS7 cells (over expressed GFP for transfection control or GFP conjugated proteins). The binding ...
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Great Pipette Aid with Steady Flow Speed
(1)
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April 03, 2023
The nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the ...
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Great Pipette for 384-Well Applications
(1)
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Thermo Fisher Scientific
March 31, 2023
The nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the ...
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Good PCR Master Mix
(1)
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New England Biolabs
March 31, 2023
Try this master mix for hard PCR application from genomic DNA. It's simple to use and the mix includes every component. The enzyme has high ...
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Fast and Great Quality RNeasy Mini Kit for RNA Isolation
(1)
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March 31, 2023
The nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the ...
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Best Results with Phusion® Hot Start Flex 2X Master Mix, Minimum Optimization
(1)
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New England Biolabs
March 31, 2023
This master mix enables researcher to minimize the time for optimization of PCR reactions, It helps to start PCR with high Tm and minimize ...
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Excellent DNA Polymerase from Takara Bio
(1)
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March 31, 2023
I used so many different DNA polymerases, but this one is perfect. They provide it in the form of 2x premix. You just need to add the ...
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Poor Signal with ROR Gamma (t) Antibody, No Distinct Positive Population
(1)
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Thermo Fisher Scientific
March 31, 2023
I'm interested in studying how diets and dietary metabolites affect host immune response, with a focus of CD4 T cells.
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Good Gelatin Coating Solution from Cell Biologics
(1)
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Cell Biologics
March 30, 2023
We study the CNS basement membrane and blood-brain barrier. Specifically the role of different CNS cell types in BBB maintenance.
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Human PDGF-BB ELISA from RayBiotech Works Well
(1)
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RayBiotech
March 30, 2023
Works well. I used to detect PDGF-BB levels in primary human brain microvascular endothelial cells. The detection levels were super high and...
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Great Recombinant Anti-Collagen IV Antibody for Immunofluorescence
(1)
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Abcam
March 30, 2023
Collagen IV antibody staining was used to identify BBB microvessels in cerebellum of mice with respiratory disease. Leakage of blood ...
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Reliable Culture Kit for Dendritic Cells
(1)
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March 30, 2023
The ImmunoCult differentiation kit can be used to reliably differentiate human monocytes to dendritic cells.
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Strong Signal with Ki-67 Monoclonal Antibody in Proliferating Cells
(1)
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March 30, 2023
I'm interested in studying how diets influence host immune responses against skin inflammation with a focus on CD4 T cell compartment.
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Novus Biologicals' PDGF-B Antibody Works Well
(1)
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Novus Biologicals, a Bio-Techne Brand
March 30, 2023
The detection level of PDGF-B antibody in the cell lysate of primary human brain microvascular endothelial cells were good.
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Great GAMT Monoclonal Antibody for Western Blot
(1)
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Proteintech Group Inc
March 29, 2023
Great antibody for western blotting. There were a few non specific bands but used for the first time and could clearly see the GAMT band ...
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Easy to Use Annexin V Apoptosis Staining Kit
(1)
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Abcam
March 29, 2023
I used this Annexin V-FITC Apoptosis Staining / Detection Kit in several of my projects to detect cell apoptosis or necrosis. The kit worked...
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Highly Efficient Human TFAM Antibody
(1)
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Cell Signaling Technology
March 29, 2023
The antibody sensitivity for Western blot analysis is exceptionally high in human cells, including mesenchymal stem cells, HUVECs, and some ...
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Great DAPI for Immunocytochemistry
(1)
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BioLegend
March 28, 2023
I use it for nuclear staining of HEK293 and HeLa cells. Good brightness and super easy to use it, also the amount needed is really really ...
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Intuitive Cytokine Array from RayBiotech
(1)
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RayBiotech
March 28, 2023
This array was used with culture media from endothelial and cardiac cultures and successfully allowed quantification of angiogenesis ...
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Amazing GAPDH Antibody from Proteintech
(1)
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Proteintech Group Inc
March 28, 2023
We use it mainly in HEK293 and COS7 cells. So far, there were no problems at all, the binding is very strong and the bands are really ...
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Poor Golgi Staining in Endothelial Cells
(1)
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Novus Biologicals, a Bio-Techne Brand
March 28, 2023
Primary mouse brain endothelial cells were stained with 58K Golgi protein antibody to differentiate secreted proteins in Golgi apparatus and...
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Great ZO-1 Antibody for Immunofluorescence
(1)
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Thermo Fisher Scientific
March 28, 2023
Primary endothelial cells cultured from mouse cortical tissue were stained with ZO-1 antibody to assess tight junction stability in vitro.
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Good Anti-IL-6 Antibody for Cytokine
(1)
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Abcam
March 28, 2023
Antibody used to detect inflammation. Works well in IHC on the brain of mice. Standard protocol, some optimisation needed. Dilution low ...
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Great Mouse Podocalyxin Antibody for Immunofluorescence
(1)
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R&D Systems, a Bio-Techne Brand
March 27, 2023
Blood vessels in mouse cerebellum were identified by immunofluorescence by staining for podocalyxin. Leakage of fibrinogen into the brain ...
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Affordable, Good Kit for Gel/PCR Purification
(1)
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March 27, 2023
I use the kit daily for Gel / PCR purifications. I think it is a good kit since it has a short and easy protocol and the loss of our ...
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Good IRAK4 Antibody for Western Blot
(1)
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BD Biosciences
March 27, 2023
We detected IRAK-4 expression in IRAK-4-overexpressed HEK293 cells via western blot. We used 30ug/well protein, however, the bands can even ...
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A Decent, Reliable Sanger Sequencing Service
(1)
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March 27, 2023
We are interested in studying the mechanisms responsible for ensuring T cell self tolerance that rely on thyme and extra-thymic Aire ...
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