Reliable and Reproducible Ly-6A/E (Sca-1) Antibody from BioLegend

May 17, 2023

University of Utah

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Pacific Blue™ anti-mouse Ly-6A/E (Sca-1) Antibody

Catalog Number:

122520

Clone Number:

E13-161.7

Info:Supplier Page View Product Specs

Browse Similar Products: Ly6a Antibodies

I use this antibody to identify fetal hematopoietic stem and progenitor populations during embryogenesis. This antibody reliably stains populations of interest within the fetal liver.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine HSPC

Primary Incubation

1:200 dilution, incubated on ice for 20 minutes, protected from light.

Blocking Agent

CD16/32 PGP

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

This antibody tags Sca1 positive cells reproducibly in the fetal liver. Plot shows LSK positive cells at embryonic day E14.5 in the fetal liver at steady state.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Reliable and easy to use.

The Bad

N/A

The Bottom Line

Great antibody overall.

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