IgG2b Isotype Control Does its Job Well

May 16, 2023

Stanford

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioXCell

Product Name:

InVivoMAb rat IgG2b isotype control, anti-keyhole limpet hemocyanin

Catalog Number:

BE0090

Clone Number:

LTF-2

We wanted to deplete CD8+ T cells in tumor-bearing mice as part of an experiment. As a control, we used the current antibody to mimic antibody infusion without depleting CD8+ T cells.

Experimental Design and Results Summary

Applications

Cell depletion control

Sample

Mouse blood

Primary Incubation

N/A

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

As a control, the isotype antibody was useful in that it didn't deplete CD8+ T cells. It also did not alter tumor growth by any noticeable amount, and therefore, served as a good control.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

The antibody serves as a great control.

The Bad

None to mention.

The Bottom Line

The antibody does its job as an isotype control.

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