Great PDPK1(Tyr373 + Tyr376) Antibody for Western Blotting

May 05, 2023

Babraham Institute

Overall

Quality of Results

Ease-of-Optimization

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Company:

Bioss Inc.

Product Name:

PDPK1(Tyr373 + Tyr376) Antibody

Catalog Number:

BS-3017R

Clone Number:

N/A

Good antibody for western blotting - very little background noise, clear signal. Used BSA to block and added 1:1000 dilution of antibody overnight at 4 degrees.

Experimental Design and Results Summary

Applications

Western Blot

Sample

MCF10a

Primary Incubation

1:1000 in 5% BSA in TBST for 1 hour at room temperature

Blocking Agent

5% BSA in TBST

Secondary Incubation

1:2000 in 5% BSA in TBST for 1 hour at room temperature

Tertiary Incubation

N/A

Detection

ECL western blotting reagent

Results Summary

Good antibody for western blotting - very little background noise, clear signal. Used BSA to block and added 1:1000 dilution of antibody overnight at 4 degrees.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very clear signal.

The Bad

Quite expensive.

The Bottom Line

Would buy again!

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