Great IRP1 Antibody for Western Blotting

May 10, 2023

Neurosurgery
Penn State College of Medicine
Research Intern

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Company:

Cell Signaling Technology

Product Name:

IRP1 Antibody

Catalog Number:

20272

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: IRP1 Antibodies

I used this antibody to indirectly measure the labile iron pool. When intracellular iron increases, IRP1 is degraded.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human astrocytes

Primary Incubation

1:1000 overnight BSA

Blocking Agent

BSA

Secondary Incubation

1 hr, milk

Tertiary Incubation

N/A

Detection

ECL

Results Summary

Using this antibody allowed me to show that my treatment decreased IRP1 and thus increased intracellular iron levels. I followed this up with a functional assay.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works well

The Bad

Nothing

The Bottom Line

I recommend it.

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