Epredia
GFAP (Glial Fibrillary Acidic Protein)
RB087A
Gfap protein from cow brain
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This antibody works on multiple species effectively. I've used it on cox, cat, dog, sheep, and rat brains. I got very satisfying results. It is very useful for inflammatory experiments on rat brain. It specifically stains astrocytes.
Immunohistochemistry
Cow brain
1/50
BSA, 1%
Anti-rabbit IgG, 1/50 dilution, RT
Peroxidase-streptavidin complex (1 hours at RT) and then AEC chromogen, RT 20'
ABC-peroxidase technique
I'm using it on mostly rat brains. But sometimes it helps differential diagnosis of animal brain tumors. No need complicated AR techniques. Standart technique of AR (citrate buffer pH 6.0) is enough. İmage legend: GFAP positives cells are astrocytes, cow brain.
N/A
Gill's hemalum as a background staining.
Easy to use, specific for multiple species.
Nope
Antibody is working on multiple species with strong and specific signals.