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Date Published
Anti-ULK2 Antibody Works Great for Western Blotting
(1)
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BosterBio
May 01, 2023
Used for western blotting. The antibody worked good, used skim milk for blocking. It was used for investigating silenced HEK293T cells.
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The Best in Experienced Hands (TUNEL Technique)
(1)
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Roche
May 01, 2023
A great KIT for imaging end-stage apoptosis in tissues or cells (TUNEL technique). It gives very good results in tissues that have been ...
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CD10 Polyclonal Antibody from ProteinTech Works Fine
(1)
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Proteintech Group Inc
May 01, 2023
I used this to measure the levels of CD10. This protein is also known by NEP-1 and is an Amyloid-beta degrading enzyme. I used this to ...
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Good GAPDH Antibody for Housekeeping Gene
(1)
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Cell Signaling Technology
May 01, 2023
Our regular study for western blot doing cell culture and mouse tissue samples. We used GAPDH for good control house keeping gene.
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Just an OK CD80 Antibody for Flow Cytometry
(1)
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BD Biosciences
May 01, 2023
I use this antibody to distinguish CD80+ and CD80- subpopulations in murine lymph nodes via flow cytometry. It doesn't work that well and it...
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Good IRP2 Antibody to Measure Labile Iron Pool
(1)
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Cell Signaling Technology
May 01, 2023
I used this as a marker for the labile iron pool within cells. Cellular iron levels modulate IRP2 and IRP1 levels - though usually only one ...
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Perfect SDS-PAGE Running Buffer
(1)
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Bio-Rad
May 01, 2023
SDS to proteins causes them to lose their higher order structures and become linear. SDS is anionic it binds to all the positive charges on ...
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Good APP Antibody for Western Blot
(1)
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Proteintech Group Inc
April 28, 2023
I used this to determine if my treatment changed APP levels. APP is the precursor to amyloid beta and plays a vital role in iron export.
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Just OK Antibody for Flow Cytometry
(1)
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BioLegend
April 28, 2023
I use this antibody in my regular panel to detect ICOSL positivity in murine lymphocyte populations by flow cytometry. It is still pretty ...
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Good ReLeSR™ Reagent for Dissociate IPS into Single Cell
(1)
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April 28, 2023
Using ReLeSR™ is a reagent to dissociate IPS cells. Cells can be dissociated into single cell, which facilitates further cell sorting.
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Good CD45 Antibody for Flow Cytometry in Murine Lymphocytes
(1)
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BioLegend
April 28, 2023
I use this antibody in my regular flow cytometry panel to detect CD45+ and negative populations within murine secondary lymphoid organs.
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Excellent LysoTracker™ Red DND-99
(1)
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Thermo Fisher Scientific
April 28, 2023
I used this product for staining of lysosomes on A549, MRC5 and SHSY5Y cells. I used a dilution factor of 1:1000 after vendor's recommended ...
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Stable Reagents for Duo Set ELISA
(1)
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R&D Systems, a Bio-Techne Brand
April 28, 2023
This ancillary reagents kits are very stable and used in most of the assays such as IL-1ß, TNF-a, etc in serum and body fluids.
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Fairly Good CD127 Antibody for Flow Cytometry
(1)
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BioLegend
April 27, 2023
I use this antibody to detect CD127 positivity in murine lymphocytes via flow cytometry. It works fairly well as it has strong signal, but ...
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Excellent Mitochondrial Superoxide Indicators from Thermo Fisher
(1)
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Thermo Fisher Scientific
April 27, 2023
I used this product for staining of mitochondria related ROS on A549, MRC5 and SHSY5Y cells. I used a dilution factor of 1:1000 in serum ...
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Good Cav-1 Antibody for Immunofluorescence
(1)
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Thermo Fisher Scientific
April 27, 2023
Expression of Caveolin-1 on brain endothelial cells increases during inflammation and is associated with increased BBB permeability. We ...
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Good Mitochondrial Marker from Proteintech
(1)
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Proteintech Group Inc
April 27, 2023
I used this to detect mitochondria in a sample. TOM20 is a protein on the outer membrane of mitochondria. It is a marker of mitochondrial ...
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Good Siglec F Antibody for Flow Cytometry
(1)
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Thermo Fisher Scientific
April 27, 2023
I use this antibody to identify eosinophils in murine lymphoid tissue. I usually gate these out first as eosinophils are auto fluorescent by...
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Good Actin Antibody for Western Blot Control
(1)
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MilliporeSigma
April 27, 2023
I used this as a loading control for western blots. It worked very well since I was looking at proteins of higher and lower molecular ...
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Anti-Ferritin Heavy Chain Antibody Did Not Work for Me
(1)
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Abcam
April 26, 2023
Ferritin is an iron storage protein. It is often used as a marker of cellular iron content. This antibody however did not work in my cells ...
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Affordable Dual Luciferase Reagent Kit
(1)
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April 26, 2023
This kit provides reagents for a two-step dual luciferase assay. It is inexpensive and reliable for Firefly and Renilla systems.
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Key DMEM/F-12 Medium in iPSC Culture
(1)
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Thermo Fisher Scientific
April 26, 2023
This medium is used for both the coating of Matrigel plates and the differentiation of induced pluripotent stem cells into astrocytes.
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Good Smooth Muscle Actin Antibody for Multiple Species on IHC Staining
(1)
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Santa Cruz Biotechnology, Inc.
April 26, 2023
I have tested this antibody in many animal species immunohistochemically. It works in cats, dogs, rats, humans, sheep, cattle and buffaloes....
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Good CD326 Antibody for Flow Cytometry
(1)
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BD Biosciences
April 26, 2023
I use this in my regular panel to detect EpCAM+ murine lymphocytes and it has good signal and good separation.
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OK CD11b Antibody for Flow Cytometry of Murine Lymphocytes
(1)
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BioLegend
April 26, 2023
I use this antibody to mark CD11b positivity in murine secondary lymphoid tissue. The signal is strong, and the separation is just OK.
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Chloroform-d Works Well for NMR Spectra
(1)
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MilliporeSigma
April 25, 2023
After the end of any reaction, there are several methods that can be used to check if it is gone to the right way or not. One of this ...
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Excellent Acti-Stain 670 Phalloidin
(1)
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April 25, 2023
I used this product for staining of cytoskeleton on A549, MRC5 and SHSY5Y cells. I used a dilution factor of 1:25 for 30 minutes at room ...
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Excellent Human Endothelial SFM for Endothelial Cell Culture
(1)
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Thermo Fisher Scientific
April 25, 2023
This medium is used to maintain endothelial cells. Specifically, it is used in the protocol to differentiate endothelial cells from iPSCs.
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Recommended Alexa Fluor™ 488 Phalloidin from Thermo Fisher
(1)
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Thermo Fisher Scientific
April 25, 2023
I used this product for staining of cytoskeleton on A549, MRC5 and SHSY5Y cells. I used a dilution factor of 1:20 for 40 minutes at room ...
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Essential Service for the Immunologist Tool Box
(1)
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April 25, 2023
Although FlowJo is a bit difficult to learn at first, it is an essential tool for every immunologist to analyze flow cytometry data. In ...
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Effective Tetrahydrofuran for Dry Synthesis
(1)
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Thermo Fisher Scientific
April 25, 2023
I used the THF extra dry at a C=0.5 M for the dehydrogenation of a secondary amide. The reaction worked fast, It needed 30 minutes at -20 °C...
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Ready to Use 1 kb DNA Ladder with Clear Bands
(1)
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Thermo Fisher Scientific
April 24, 2023
In our lab we used for a DNA Gel electrophoresis on a PCR product of 500bp. Clear results and easy detection of the bands thanks to the ...
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Good Medium for iPSC Differnetiation
(1)
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Thermo Fisher Scientific
April 24, 2023
This medium is used in the differentiation of induced pluripotent stem cells to endothelial cells. The medium is used to prime the iPSCs for...
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Great Luciferase-Based Human IFN-beta ELISA
(1)
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April 24, 2023
Quick and easy-to-use human IFN-beta ELISA. It gave robust and reproducible results. Economical, too.
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TeSR E5/E6 Medium Works Well for iPSC Differentiation
(1)
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April 24, 2023
This medium is used in the differentiation of induced pluripotent stem cells to endothelial cells. The medium is used to prime the iPSCs for...
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Good Hot-Start PCR Master Mix
(1)
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April 24, 2023
Use this master mix to amplify human genomic DNA. It includes all of components and just needs DNA and water. It works very well, and almost...
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Great Cytochrome C Antibody for Western Blotting
(1)
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Proteintech Group Inc
April 24, 2023
Cytochrome C is an iron rich complex in mitochondria. This antibody works beautifully to detect the small protein.
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Great TRIzol™ LS Reagent for RNA Extraction
(1)
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Thermo Fisher Scientific
April 24, 2023
This Trizol reagent have been used in a cancer related project. We use it for isolation of RNA from both cell line and tissue for PCR.
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Nice TNF Alpha Mouse Uncoated ELISA Kit
(1)
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Thermo Fisher Scientific
April 21, 2023
We have been used this ELISA kit for cancer related projects. We want to detect TNF alpha cytokine release in bone marrow derived dendritic ...
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Clean and Sharp Immunohistochemical Staining with Beta Catenin Antibody
(1)
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Abcam
April 21, 2023
Although it is a polyclonal antibody, it gives a specific and strong signal in immunohistochemical staining. Background staining is close to...
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Great PHD3 Antibody from Novus Biologicals
(1)
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Novus Biologicals, a Bio-Techne Brand
April 21, 2023
We used this PHD3 antibody in a study of investigating cellular metabolism in Tissue-resident alveolar macrophages affected by hypoxia.
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Good Essential 8™ Medium for iPSC Culture
(1)
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Thermo Fisher Scientific
April 21, 2023
This media is used for induced pluripotent stem cell maintenance and culture. I was this medium for that exact purpose.
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Non-Enzymatic Alternative to Trypsin
(1)
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Corning Life Sciences
April 21, 2023
The Cellstripper product can be used to release adherent cells without any enzyme activity. This product is ideally suited to fragile cell ...
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100 bp DNA Ladder Easy to Detect and Clear Bands
(1)
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Thermo Fisher Scientific
April 21, 2023
We did use this ladder to detect a ds DNA in the range of 100 bp to 1,000 bp on agarose gel (1-3%) plus ethidium bromide. The ladder needs ...
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Good PerCP/Cyanine5.5 Anti-Mouse Ly-6C for Flow Cytometry
(1)
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BioLegend
April 20, 2023
I use this antibody in my regular flow cytometry panel to identify Ly6C+ murine lymphocytes. It has good signal and good separation.
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Great Human Transferrin Biotin to Identify Exogenous Tf
(1)
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Rockland Immunochemicals, Inc.
April 20, 2023
I used this to identify exogenous Tf in a solution. Importantly, this is apo-Tf (iron free). Since I study differences between apo- and holo...
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Great Pierce Lane Marker Reducing Sample Buffer for Western Blotting
(1)
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Thermo Fisher Scientific
April 20, 2023
I use this for all of my western blotting. It nicely reduces the sample for loading. The 5x concentration allows for increased protein ...
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Very Sensitive and Specific Amyloid beta 42 Human ELISA Kit
(1)
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Thermo Fisher Scientific
April 20, 2023
This ELISA is to measure the amount of full length amyloid-beta42 in a sample. It can be used on cells, cell media, tissue, or human CSF.
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Laemmli Sample Buffer Works Well for Immediate Western Blotting
(1)
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Bio-Rad
April 20, 2023
I often study cells grown in bi-chamber plates, leading to much difficultly in collecting them for western blotting. In those circumstances,...
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Usable CD3e Antibody but Not Great Separation
(1)
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Bio-Rad
April 20, 2023
I use this antibody in my regular panel for mouse lymphocytes. I don't get super great separation between CD3+ and CD3- populations, but it ...
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Analytical Equipment
Animal Handling Equipment
Antibodies
Balance / Scale
Beads
Biomolecules
Blotting Equipment
Blotting Membranes and Papers
Camera
Cell Analysis Equipment
Cell Sorting Equipment
Cells and Strains
Centrifuge
Chemicals and Reagents
Chromatography Equipment
Columns
Concentrator
Cooler
Counter
Cytometer
Electrophoresis Equipment
Electroporation Equipment
ELISA Kits
Enzymes
Fluorescence Spectrophotometer
Freezer
Homogenizer
Imaging Equipment
Incubator
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Kits
Labels / Dyes / Stains
Ladders and Markers
Light Sources
Liquid Handling Equipment
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Master Mixes
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Microscopes
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PCR Equipment
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Plate Reader
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Primary Cells
Proteins
Purification Equipment
Refrigerator / Freezer
Rocker
Sample Prep Equipment
Scanners
Services
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Slides
Software
Sonicator
Spectrometer
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Substrates
Transfection Equipment
Vacuum Manifolds
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Applications
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Western Blot
Flow Cytometry
ELISA
Immunofluorescence
Immunoprecipitation
Immunocytochemistry
Immunohistochemistry
Block/Neutralize/Inhibit
Cell Isolation
Dot Blot
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Purification
Staining
Cell Culture
DNA Purification
PCR
RNA Analysis
RNA Purification
qPCR
Clean-up
Microscopy
Cloning
RT-PCR
cDNA Synthesis
Transfection
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Cell Counting
Protein Purification
Next Gen Sequencing (NGS)
Library Construction
Genotyping
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Protein Assay
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Cell Lysis
Multiplex
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RNA-Seq
Live Cell Imaging
Cell Differentiation
Ligation
Concentration
HPLC
Sequencing
Chromatography
Mass Spectrometry
Labeling
Microarray
Mutagenesis
FISH
CRISPR
TUNEL Assay
In Vivo Imaging
EMSA
Cell Fractionation
Immunoassay
In Vitro Transcription
ChIP-Seq
Northern Blot
3D Imaging
RNAi
ELISPOT
PCR Cloning
Particle Analysis
Southern Blot
Protein Modification
DNA Analysis
Expression
Cell-Based Assay
Gene Editing
In Vivo Testing
Protein Labeling
Other
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Human
Mouse
Rat
Non-Human Primate
Rabbit
Sheep
Hamster
Chicken
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C. elegans/Worm
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Arabidopsis
Yeast/Fungi
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Other Invertebrate
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Drosophila
Goat
Rhesus
Tissue
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Adipose
Aorta
Artery
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Blood
Blood Vessel
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Brain
Breast
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Eye
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Gingiva
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Hippocampus
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In Vivo
Intestine
Kidney
Large Intestine
Liver
Lung
Lymph Node
Mammary Gland
Mouth
Muscle
Ovary
Pancreas
Peripheral Blood
Peritoneum
Placenta
Prostate
Rectum
Retina
Skeletal Muscle
Skin
Small Intestine
Spinal Cord
Spleen
Stomach
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Tail
Teeth
Testis
Thymus
Thyroid Gland
Tonsil
Trachea
Umbilical Cord
Umbilical Vein
Uterus
Vein
Cell Type
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Adipocyte
Astrocyte
B Cell
CD8+ T Cell
Competent Cells
Dendritic Cell
Embryonic Stem Cell
Endothelial Cell
Eosinophil
Epithelial Cell
Fibroblast
Glial Cell
Granulocyte
Helper T Cell
Hematopoietic Stem Cell
Hepatocyte
HUVEC
Induced Pluripotent Stem Cell
Keratinocyte
Leukocytes
Lymphocyte
Macrophage
Mesenchymal Stem Cell
Microglia
Microvascular Endothelial Cell
Monocyte
Myoblasts
Myocyte
Neuron
Neutrophil
NK Cell
NKT Cell
Oligodendrocytes
Osteoclast
PBMC
Platelets
Progenitor Cell
Red Blood Cell
Regulatory T Cells
Skeletal Muscle Cell
Smooth Muscle Cell
Splenocyte
Stem Cell
Stromal Cell
Synoviocyte
T Cell
Thymocytes
Tumor Cells
Cell Line
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HeLa
PC3 (PC-3)
HEK293
HEK293T (HEK-293T)
CHO
Jurkat
3T3
Caco-2
HepG2
THP-1
U2OS
PC12 (PC-12)
HL60 (HL-60)
A375 (A-375)
MOLT4 (MOLT-4)
MCF-7
A431
MDA-MB-231
A549
N2a
Daudi
HCT-116
Raji
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