BioLegend
PerCP/Cyanine5.5 anti-human CD3 Antibody
344808
SK7
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We are an immunotherapy focused group with an interest in CD8 T-cells and their TCRs. For our experimental purposes, we routinely use transduced PBMCs.This antibody is used in our panel to separate our T-cell populations.
Flow Cytometry
PBMCs
1:100, 15min RT
N/A
BD LSR II
PBMCs were stained with the CD3-PerCP/Cyanine5.5 and the CD8-FITC antibodies to evaluate the percentage of the CD8 population. A clear separation is evident when stained with both these antibodies.
Easy to use, works well, reliable between batches.
None
A good antibody for staining human CD3 and the fluorochrome is quite bright, although not as strong as PE or APC.