Tenascin C Antibody from Abcam

Afyon Kocatepe University

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Recombinant Anti-Tenascin C antibody

Catalog Number:

ab108930

Clone Number:

EPR4219

Tenasin C positivity is found in both normal and pathological tissues. I usually use it in experimental inflammation studies of central nervous system. There are studies suggesting that it has prognostic importance especially in tumors of the central nervous system too.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Cerebellum

Primary Incubation

1/200, RT, 2hours

Blocking Agent

BSA, %1

Secondary Incubation

Biotinylated Anti-Rabbit IgG, 1/200

Tertiary Incubation

AEC chromogen, RT, 20'

Detection

ABC-peroxidase

Results Summary

I got excellent results using citrate buffer pH 6.0 for 20 minutes MW treatment; clean background, specific and intense positivity. In the photo you can see diffuse Tenascin C positivity in the molecular layer of the cerebellum.

DOI or PMID #

N/A

Additional Notes

Gill's hemalum used as a background staining.

Image Gallery

Summary

The Good

Great result both normal and pathological tissues.

The Bad

Nope

The Bottom Line

You can get clean, specific and intense positivity with this Tenascin C antibody.

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