Cell Signaling Technology
IκBα Antibody
4814
N/A
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Transcription factor NF-kB which is present in the cytosol are in inactive state by IκB complex proteins. Once it activated by the phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB. Followed by IκBα phosphorylation and resulting Rel-dependent activation of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Here we checked the time course of IκBα expression by chemoattractant.
Western Blot
Mouse Lysate
1:1000 prepared in 1x TBST
5% Skim milk
1:4000 prepared in 1x TBST
None
ECL
We performed western blot and detect, analyzed the IκBα expression. We found that there is a a time dependent increase of IκBα expression by chemoattractant.
This antibody works great for western blot.
This antibody works well for western blot and signal is very strong. Highly recommended.