Time Course of VARS2 Expression by Chemoattractant

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Thermo Fisher Scientific (Invitrogen)

Product Name:

VARS2 Polyclonal Antibody

Catalog Number:

PA5-64437

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: VARS Antibodies

VARS2 encodes a mitochondrial aminoacyl-tRNA synthetase. It catalyzes the mitochondrial translation and attachment of valine to tRNA(Val). If mutations occurs in this gene cause combined oxidative phosphorylation deficiency-20, which leading to early-onset mitochondrial encephalopathies. Here we checked the time course effect of VARS2 expression by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse Lysate

Primary Incubation

1:1000 in 1xTBST

Blocking Agent

5% milk in 1X TBST

Secondary Incubation

1:4000 in 1X TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We checked the VARS2 expression in mouse lysate by using western blot. We found there is no change in VARS2 expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

This antibody works for western blot.

The Bad

There is some little background and we have to use high concentration.

The Bottom Line

This antibody works for western blot. Highly recommended.

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