ThermoFisher Scientific
Pierce™ BCA Protein Assay Kit
23227
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This kit was used to measure protein content from cell lysates. A standard curve is generated with known concentrations of BSA and unknown protein concentration is measured from the standard curve.
Protein concentration measurement from absorbance values.
Cell lysate, purified proteins
Can be used in microformat in 96-well plate.
Standard curve showing absorbance at 562 nm versus increasing concentration of BSA. The absorbance was measured with a standard plate reader at 562 nm.
N/A
Quick (30 min), reproducible, most ionic and non-ionic detergents does not interfere with the assay.
None.
A reliable protein concentration estimation kit.