Efficient RNeasy Mini Kit for Fast and Simple RNA Purification

MD Anderson Cancer Center - University of Texas
Cancer System Imaging (Diagnostic Imaging Division)
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

QIAGEN

Product Name:

RNeasy Mini Kit

Catalog Number:

74104

Info: Supplier Page 

RNeasy Mini Kit allows the purification of RNA from small amounts of starting material (e.g., animal or yeast cells, mice tissues), useful for different downstream molecular biology applications, such as in our case qT-PCR. This method is a fast and easy way to purify the total RNA from small numbers of cells, in our case cells have been seeded in a concentration between 5 x 105 to 1 x 106cells in a 100 mm x 20 mm Tissue Culture dish. Once purified the RNA has been quantified at Nanodrop before the PCR. Highly recommended..

Experimental Design and Results Summary

Application

RNA purification for downstream RT-qPCR

Starting Material

Immortalized cancer cell line

Tips

N/A

Results Summary

This method is a fast and easy way to purify the total RNA from small numbers of cells, in our case cells have been seeded in a concentration between 5 x 105 to 1 x 106 cells in a 100 mm x 20 mm tissue culture dish. Once purified the RNA has been quantified via a Nanodrop before the RT-qPCR.

DOI or PMID #

N/A

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Summary

The Good

Fast, easy-to-use and yields high quality results.

The Bad

Requires optimization with starting material for best results.

The Bottom Line

Fast and easy method for the delivering of high-quality and ready-to-use total RNA for different downstream molecular biology applications.

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