Excellent Staining With MHC-II Antibody From BD Biosciences

July 29, 2023

BlueskyReddit
Wayne State University

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BD Biosciences

Product Name:

BD Horizon™ BV711 Rat Anti-Mouse I-A/I-E

Catalog Number:

563414

Clone Number:

M5/114.15.2 (also known as M5/114)

Info: Supplier Page

Browse Similar Products: Anti-I-A/I-E Antibody Products 

Great staining of MHC-II on mouse immune cells from intestinal single cell suspensions. Very bright staining.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse intestine

Primary Incubation

1:500 for 15 min at 4°C

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Fortessa LSRII

Results Summary

Mouse intestinal immune cells in single cell suspensions stained with this antibody show very strong staining of MHC-II with this antibody. Signal is very bright and positive population is very clear.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Excellent staining.

The Bad

Extremely bright.

The Bottom Line

The staining is extremely bright so use a lower concentration of the antibody. If an optimized concentration is used, the staining is very good.

Share your experience with other scientists. Write a Review! »

Join the discussion