Invitrogen™
Amplex™ Red Cholesterol Assay Kit
A12216
Info: Supplier Page
I am interested in measuring membrane cholesterol upon statin treatment from mammalian cells. I used the Amplex™ Red Cholesterol Assay Kit for measuring total cholesterol from membrane preparations.
ELISA
Cell lysates or membrane
Can avoid cholesterol esterase to get non-esterified membrane-bound cholesterol.
Membrane cholesterol content of CHO-K1 cells treated with increasing concentrations of lovastatin. Values are expressed as absolute cholesterol content in cell membranes and are normalized to protein content. Data represent means ± SEM of three independent experiments (* and *** correspond to a significant difference [P < 0.05 and P < 0.001] in membrane cholesterol content in lovastatin-treated cells relative to untreated cells).The inset shows cholesterol/phospholipid ratio (C/P, mol/mol) under these conditions. Data represent means ± SEM of three independent experiments (** and *** correspond to a significant difference [P < 0.01 and P < 0.001] in C/P ratio in lovastatin-treated cells relative to untreated cells).
https://doi.org/10.1016/j.jlr.2022.100206
Rapid assay, provides both free and esterified cholesterol, and has got a good dynamic range.
None.
This is an easy to use kit for rapid measurements of cholesterol content.