Precast Gradient Protein Gels From Invitrogen

Stanford University
Biochemistry
Postdoctoral Researcher

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Company:

Invitrogen™

Product Name:

NuPAGE™ 4 to 12%, Bis-Tris, 1.0–1.5 mm, Mini Protein Gels

Catalog Number:

NP0321BOX

Info: Supplier Page 

In my research, I work extensively with proteins and encounter a diverse range of protein sizes. To effectively analyze and separate these proteins, I utilize gradient gels in Western blotting. The gradient gel allows for the precise resolution of proteins based on their molecular weights, enabling accurate identification and quantification of various protein species in my experiments.

Experimental Design and Results Summary

Application

Protein visualization, Western blot.

Starting Material

Cell lysate, purified proteins.

Tips

Wash the wells with running buffer before loading protein sample.

Results Summary

The pre-casted gradient gel exhibits a stunning and even separation of molecular weight proteins ranging from 250 to 10 kDa, showcasing its efficiency and reliability in achieving precise protein resolution during the experiment.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Reproducible separation of protein every time. Saves time from pouring home-made gels.

The Bad

None.

The Bottom Line

These pre-cast gels provide a convenient and reliable solution for protein separation in Western blotting, allowing accurate resolution of proteins based on their sizes and facilitating efficient protein analysis in research experiments.

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