Highly Sensitive Pico PLUS Chemiluminescent Substrate

July 30, 2023

BlueskyReddit
Stanford University
Biochemistry
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Scientific™

Product Name:

SuperSignal™ West Pico PLUS Chemiluminescent Substrate

Catalog Number:

34578

Info: Supplier Page 

As a researcher, working with proteins expressed at extremely low levels has been quite challenging when conducting Western blot experiments using regular ECL reagents. However, I found a solution with the Pico PLUS Chemiluminescent Substrate. This specialized reagent offers enhanced sensitivity, allowing me to detect even the faintest protein bands on the blot, leading to more accurate and reliable results.

Experimental Design and Results Summary

Application

Western blot

Starting Material

Transferred protein on nitrocellulose/PVDF membranes bound to antibodies with HRP tag.

Tips

Optimize dilutions: Since you are working with low-expressing proteins, it's essential to optimize the substrate dilution to achieve the best signal-to-noise ratio. Test different dilutions to find the most suitable one for your specific experimental conditions.

Results Summary

Using the Pico PLUS Chemiluminescent Substrate, the Western blot successfully detected a low-abundance protein, demonstrating its high sensitivity and reliable performance, allowing for accurate visualization and quantification of the faint protein band.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

The Pico PLUS Chemiluminescent Substrate is a highly sensitive and reliable solution for detecting low-abundance proteins in Western blot experiments. Its enhanced signal-to-noise ratio ensures accurate and reproducible results, even with faint protein bands. The substrate's long-lasting signal stability allows for flexible image capture without compromising data quality.

The Bad

None.

The Bottom Line

The Pico PLUS Chemiluminescent Substrate offers highly sensitive and reliable protein detection for low-abundance samples.

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