Excellent IL-7A Antibody From BD Biosciences

Wayne State University

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BD Horizon™ BUV395 Rat Anti-Mouse IL-17A

Catalog Number:

565246

Clone Number:

TC11-18H10 (RUO)

Great staining of intracellular IL-7A in mouse immune cells. The separation of the positive population is decent.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse intestine

Primary Incubation

1:200 for 15 min at 4°C

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Fortessa LSRII

Results Summary

Excellent staining of intracellular IL17A in mouse immune cells isolated from the intestines. Strong staining presenting as a smear, but with a clear positive signal.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very good staining.

The Bad

None.

The Bottom Line

Excellent antibody requiring little compensation. Definitely consider adding if staining panel allows.

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