Antibody With Great Staining for Flow From BioLegend

July 26, 2023

Wayne State University

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 700 anti-mouse CD90.2 (Thy1.2) Antibody

Catalog Number:

105320

Clone Number:

30-H12

Great staining of mouse CD90 for flow cytometry. Very specific and strong signal giving good separation of positive and negative populations

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse intestine

Primary Incubation

1:200 for 15 min at 4°C

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

None

Detection

Fortessa LSRII

Results Summary

This antibody very specifically stains CD90 on mouse immune cells in the intestines. The signal is bright enough to get good separation of the positive population.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very good staining.

The Bad

None.

The Bottom Line

Great staining of mouse CD90 on single cell suspensions from mouse intestines. Very specific and bright signal providing good separation of positive and negative populations.

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