Excellent Staining With CD4 Antibody From BioLegend

July 28, 2023

Wayne State University

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 605™ Anti-Mouse CD4 Antibody

Catalog Number:

100451

Clone Number:

GK1.5

Great separation of the CD4 positive population from the negative when intestinal cells are stained with this antibody.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse intestine

Primary Incubation

1:200 for 15 min at 4°C

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Fortessa LSRII

Results Summary

Mouse immune cells from intestine stained with this antibody show strong and specific staining with a clear separation between positive and negative populations.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Excellent staining.

The Bad

None.

The Bottom Line

Fantastic staining of CD4 on mouse immune cells from intestinal single cell suspension. Worth adding to flow panel if colors are compatible.

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