Cheap and Simple On-Column DNase Kit

July 28, 2023

BlueskyReddit
University of Toronto
Chemical/Biomedical Engineering
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Qiagen

Product Name:

RNase-Free DNase Set

Catalog Number:

79254

Info: Supplier Page 

Total RNA was isolated from lysed cells and extracellular vesicles for qPCR and miRNA sequencing. Column-based isolation was performed, adding an on-column DNase digestion step before RNA elution to remove contaminating DNA before PCR/sequencing.

Experimental Design and Results Summary

Application

DNA removal before RNA sequencing/qPCR.

Starting Material

Total RNA, isolated using column-based kit

Tips

Follow the manufacturer protocol depending on column-based kit used.

Results Summary

RNA was successfully isolated and treated to remove DNA, with successful sequencing and PCR revealing distinct differentially expressed genes. Purity and quality of isolated RNA was generally acceptable with a few exceptions.

DOI or PMID #

N/A

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Summary

The Good

Very simple on-column digestion method, reasonably priced.

The Bad

Perhaps a risk of lower quality RNA compared to other DNase kits (such as bead-based methods).

The Bottom Line

Suitable for quick and easy DNase treatment. If sample quality or quantity is a concern, this kit may be less favourable.

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