Excellent FOXP3 Antibody for Flow Cytometry From BioLegend

July 28, 2023

BlueskyReddit
Wayne State University

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Company:

BioLegend

Product Name:

Pacific Blue™ Anti-Mouse FOXP3 Antibody

Catalog Number:

126410

Clone Number:

MF-14

Great staining of FOXP3 in mouse immune cells from the intestines. Very clear signal and great separation of positive population.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse intestine

Primary Incubation

1:200 for 15 min at 4C

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Fortessa LSRII

Results Summary

Mouse immune cells from mouse intestinal single cell suspensions stained with this antibody show great separation of positive and negative populations.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Excellent staining.

The Bad

None.

The Bottom Line

Great staining of mouse FOXP3 in intestinal immune cells after fix/perm. Very useful antibody for looking at Tregs due to good separation of populations.

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