Excellent CD8a Antibody From BioLegend

July 28, 2023

BlueskyReddit
Wayne State University

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

Brilliant Violet 650™ Anti-Mouse CD8a Antibody

Catalog Number:

100742

Clone Number:

53-6.7

Very strong and specific staining of CD8a in mouse T-cells. Great separation of positive and negative populations.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse intestine

Primary Incubation

1:200 for 15 min at 4°C

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Fortessa LSRII

Results Summary

Single cell suspension of mouse immune cells from intestine stained with this antibody shows excellent separation of the positive population from the negative.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Excellent staining.

The Bad

None.

The Bottom Line

Very strong staining of CD8a in mouse intestinal T-cells. Great for separating out the CD8 T-cells by flow cytometry.

Share your experience with other scientists. Write a Review! »

Join the discussion