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Date Published
Great Anti-Human CD45 Antibody
(1)
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BD Biosciences
January 17, 2019
PBMC of a healthy donor were stained with viability dye and anti-human CD45-BUV (clone HIB30) at varying concentrations. The antibody gives ...
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Does Not Work Against Tdtomato For Western Blot, Alright For Immunofluorescence
(1)
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Rockland Immunochemicals, Inc.
January 17, 2019
This was tested by IF and Western blot against TdTomato. Results of western blot showed that there was no recognition of any bands. Results ...
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Trans-Blot Turbo Transfer System
(1)
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Bio-Rad
January 17, 2019
Extremely fast blot transfer. Cuts down western blot preparation time by a significant amount. The bottom line - a great alternative to the ...
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The Antibody Stains IgM+ B Cells Very Well
(1)
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BD Biosciences
January 17, 2019
We wanted to study B cells in the diseased vs healthy state, so we used this antibody to stain IgM+ B cells in the peritoneum. The antibody ...
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Excellent Kit for In Vivo Analysis
(1)
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Promega
January 17, 2019
Cancer cells are transfected with the plasmid that encodes for the enzyme luciferase. After introducing the cells into the mouse models, the...
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H2DCFDA Detects Intracellular ROS Levels in 15d-PGJ2 Treated Cardiomyocytes
(1)
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Tocris Bioscience, a Bio-Techne Brand
January 17, 2019
HL-1 cardiomyocytes were stimulated with 15d-PGJ2 up to 60 min to follow intracellular ROS levels using H2DCFDA.
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Lipofectamine 3000-Enhanced Transient Transfection
(1)
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Thermo Fisher Scientific
January 17, 2019
Similar to earlier generations, lipo3000 is used to transfect a broad spectrum of cell lines such HEK293T with plasmid DNA. Transfection of ...
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PE/Cy7 Anti-Mouse CD94 Antibody
(1)
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BioLegend
January 17, 2019
I am studying molecular anomalies in signaling pathways leading to cancers and the development of anti-cancer immune therapies. This ...
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Excellent Immunostaining of Cortical Neurons
(1)
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Santa Cruz Biotechnology, Inc.
January 17, 2019
The general aim of our research is to study neurogenesis in rodents with maternal immune activation. The specific goal of this experiment ...
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Good Antibody for p62 Detection
(1)
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Cell Signaling Technology
January 16, 2019
Cell lysates are collected from different stimuli and western blot analysis is carried out to determine changes in p62 expression. The ...
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The Antibody Stains CD23+ B Cells Very Well
(1)
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BD Biosciences
January 16, 2019
We wanted to study the B cell population in the diseased vs healthy state, so we used this antibody to stain CD23 in the peritoneum. The ...
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Cell Media Thermoshaker Pro
(1)
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January 16, 2019
Excellent piece of machinery. This was an upgrade in our lab from the usual heating-only thermoshakers. It both heats and cools very quickly...
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Good Antibody for Siglec-1 Detection on Tissue Sections.
(1)
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Novus Biologicals, a Bio-Techne Brand
January 16, 2019
This antibody was used in a study that investigated inflammatory status in small intestine. The antibody reviewed here provided reasonable ...
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Good Mouse Ki67 Antibody
(1)
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BioLegend
January 16, 2019
Our lab studies the function of T cells in an influenza model in mice. One of our interests is studying the proliferation of these T cells ...
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Great Flow Antibody to Detect IFNg Secretion in T Cells
(1)
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Thermo Fisher Scientific
January 16, 2019
As an immunologist, we assess CD8 T cell cytokine secretion via flow cytometry. We analyze interferon gamma secretion (IFNg) since this ...
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Easily Mounts Slides With No Bubbles, Preserves Antibody, Curing Is Not Very Good
(1)
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Thermo Fisher Scientific
January 16, 2019
This is used to mount all slides in our lab. It retains good signal from the antibody for about a week after which it starts to degrade. ...
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Brilliant Violet 785 Anti-Mouse/Human CD44 Antibody
(1)
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BioLegend
January 16, 2019
I am studying molecular anomalies in signaling pathways leading to cancers and the development of anti-cancer immune therapies. This ...
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TC-E 5007 Impairs Ca2+ Transient Relaxation
(1)
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Tocris Bioscience, a Bio-Techne Brand
January 16, 2019
Representative Ca2+ transient traces of control and a TC-E 5007 (2 µM and 2 hr for all)-treated mouse ventricular cardiomyocytes ...
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Bright Staining of TCRgd
(1)
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Miltenyi Biotec
January 16, 2019
PBMC of a healthy donor were stained for viability dye and anti-human TCRgd-FITC to detect TCRgd-expressing cells. The antibody has great ...
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The Antibody Stains CXCR5+ Cells in the Lymph Nodes Very Well
(1)
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BioLegend
January 15, 2019
We wanted to study CXCR5+ cells in the diseased vs healthy state, so we used this antibody to identify CXCR5+ cells in the lymph node. The ...
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Great Antibody to Detect Granzyme B Expression in T Cells
(1)
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BioLegend
January 15, 2019
We routinely assess cytolytic activity in CD8 T cells upon acute infections. Granzyme B is a canonical marker for this activity using flow ...
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Staining Specific to GM1 of Cell Membrane
(1)
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Thermo Fisher Scientific
January 15, 2019
This reagent is used to stain the GM1 of the cell membrane and subsequently used for fluorescence microscopy imaging to see if there is any ...
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Works to Stimulate NF-KB In Endothelial Cells, but Expensive
(1)
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January 15, 2019
This was used along with LPS to measure the inflammatory response in endothelial cells as measured by E-selectin mRNA levels. Works ...
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Anti-S744,748-PKD1 Antibody for Western Blot
(1)
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Cell Signaling Technology
January 15, 2019
lysates were made by adding lysis buffer [1% SDS, 10 mmol/L Tris-Cl (pH 7.6), 20 µg/µL aprotinin, 20 µg/µL leupeptin, and 1 mmol/L 4-(2-...
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Very Bright Mouse CD11a Antibody
(1)
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BD Biosciences
January 15, 2019
Our lab studies the function of T cells in an influenza model in mice. One of our target T cell groups are those expressing CD11a. In order ...
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Acetylcholine Chloride Induces Aortic Ring Relaxation
(1)
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Tocris Bioscience, a Bio-Techne Brand
January 15, 2019
C57BL6 mice were administered with LPS (5 mg/kg) alone or in combination with Empagliflozin (1 µM) for 16 hr. Afterwards mice were ...
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APC Anti-Mouse DLL4 Antibody
(1)
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BioLegend
January 15, 2019
I am studying molecular anomalies in signaling pathways leading to cancers and the development of anti-cancer immune therapies. This ...
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Excellent Nuclear Stain from Life Technologies
(1)
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Thermo Fisher Scientific
January 15, 2019
In the present study, rat kidney tissue was harvested, micro sections were made and immunostained for pErk 1/2 proteins. Same tissue ...
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Streptavidin-BV711
(1)
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BD Biosciences
January 15, 2019
This product is good for clear detection of biotinylated antibodies.
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Strong Detection Of Heca-452 Antibody Clone Bound To Human Cells
(1)
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Thermo Fisher Scientific
January 14, 2019
This antibody was used to detect the Heca-452 Rat IgM clone binding to human MDA-MB-231 cells. Antibody works great and at a good price.
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Anti-S916-PKD1 Antibody for Western Blot
(1)
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Cell Signaling Technology
January 14, 2019
The expression levels of phospho-S916-PKD1 were detected. Western blot findings using this antibody have been published in a paper.
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The Antibody Identifies CD4+ Cells Very Well
(1)
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BioLegend
January 14, 2019
We wanted to study CD4+ T cells in the diseased vs healthy state, so we used the antibody to stain CD4+ T cells in the lymph nodes. The ...
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Very Bright Antibody to Detect CD90.1 Congenic Marker on T Cells
(1)
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BioLegend
January 14, 2019
To analyze cell-intrinsic properties of certain proteins, we routinely perform adoptive transfer experiments of congenically different ...
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Clear Detection of CD19+ Lymphocytes
(1)
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BioLegend
January 14, 2019
To detect CD19+ B lymphocytes, PBMC were isolated from peripheral blood of a healthy donor. PBMC were stained with viability dye and anti-...
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Phlorizin Inhibits LPS-Induced iNOS in HL-1 Cells
(1)
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Tocris Bioscience, a Bio-Techne Brand
January 14, 2019
HL-1 cardiomyocytes were stimulated with Phlorizin (0-200 µM) alone or in combination with LPS (1 µg/ml) for 8 hr to follow iNOS ...
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The Best Shaking Incubator
(1)
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Amerex Instruments, Inc.
January 14, 2019
I teach at a small liberal arts college and am the only microbiologist in my department. In addition to teaching microbiology with a weekly ...
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Hard to Obtain Nice Bands for Western Blot
(1)
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Cell Signaling Technology
January 14, 2019
Western blot for changes in LAMP protein expression levels after carrying out different conditions in the cell culture. The bands produced ...
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Good TCF1/TCF7 Antibody
(1)
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Cell Signaling Technology
January 14, 2019
Our lab studies the function of T cells in an influenza model in mice. One of our interests is to see how TCF1 expression may change due to ...
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APC/Cy7 Anti-Mouse CD86 Antibody
(1)
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BioLegend
January 14, 2019
I am studying molecular anomalies in signaling pathways leading to cancers and the development of anti-cancer immune therapies. This ...
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Gives Fast and Accurate qPCR Results
(1)
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Thermo Fisher Scientific
January 11, 2019
I use this on a regular basis to measure gene expression in bacterial cells. I make cDNA from mRNA, and after mixing with primers, probe, ...
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Great HSP60 Antibody from GeneTex for Western Blot
(1)
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GeneTex
January 11, 2019
This antibody was used to detect HSP60 expression in iPSCs by Western blot analysis. The antibody has high sensitivity and works perfectly.
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Excellent pJNK1/2/3 Antibody from Abcam
(1)
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Abcam
January 11, 2019
Male albino rats were fed with or without a high quantity of fat (42%) in the diet for 3 months to induce atherosclerotic lesions. Rats fed ...
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PE Anti-Mouse CD170 (Siglec-F) Antibody
(1)
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BioLegend
January 11, 2019
I am studying molecular anomalies in signaling pathways leading to cancers and the development of anti-cancer immune therapies. This ...
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Excellent Anti-Mouse CD4-APC Monoclonal Antibody for Flow Cytometry
(1)
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Thermo Fisher Scientific
January 11, 2019
CD4+ T-cells differentiate into different effector subsets after exposure to antigen. We routinely do staining of mice splenocytes with CD4-...
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Used to clone 86 genes, zero errors detected by sequencing
(1)
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Thermo Fisher Scientific
January 11, 2019
This polymerase has such a low error rate it is unquantifiable. Used for annealing reactions between 67-72 C, and extension at 72 C.
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CD80 to Measure Dendritic Cell Activation
(1)
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BioLegend
January 11, 2019
Anti-human CD80 BV421 is an excellent bright antibody to measure activation of human peripheral blood DC after pIC stimulation.
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PDTC Inhibits HOCl-LDL-Induced CaMKII Oxidation
(1)
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Tocris Bioscience, a Bio-Techne Brand
January 11, 2019
HL-1 cardiomyocytes were pre-treated with Pyrrolidinedithiocarbamate (PDTC, 1 mM), Tempol (1 mM) or N-Acetylcysteine (NAC, 1 mM) for 30 min ...
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Great Antibody to Detect PD1 Expression on T Cells
(1)
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BioLegend
January 11, 2019
As a cancer immunology lab, we assess T cell exhaustion by analyzing surface expression of checkpoint inhibitors such as PD1. PD1 expression...
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The Antibody Identifies T Cells Very Well
(1)
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BioLegend
January 11, 2019
We wanted to study T cells in the diseased vs healthy state, so we used this antibody to identify T cells in the spleen. The antibody works ...
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Brilliant Violet 421 Anti-Mouse CD45 Antibody
(1)
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BioLegend
January 10, 2019
I am studying molecular anomalies in signaling pathways leading to cancers and the development of anti-cancer immune therapies. This ...
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