Usable Streptavidin BUV805 Antibody

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BUV805 Streptavidin

Catalog Number:

564923

Browse Similar Products: Streptavidin Flow Cytometry Antibodies

B cells have been shown to play a major role in pathogenesis of autoimmune diseases through presentation of antigen to T cells, production of autoantibodies and inflammatory cytokines that modulate immune responses. On the other hand, B cells act as a regulator through production of regulatory cytokine IL-10, inhibiting pathogenic T cells responses. In this part of the project we wanted to characterize a subset of B cell that express CD1d. It is a small population so using CD1d Biot SA to have a good signal was tried. In parallel, we tried to expand the panel using a SA labeled with a new fluorochrome.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

C57BL/6 spleen

Primary Incubation

CD1d biotin, 20 min at 4C

Blocking Agent

Fc block (2.4G2 clone)

Secondary Incubation

SA BUV805

Tertiary Incubation

NA

Detection

Flow Cytometer with UV laser

Results Summary

We titrated the SA BUV805 and found that the SA can be use at 1:450.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

It works

The Bad

Is expensive

The Bottom Line

SA BUV805 can be used but it is not ideal.

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