BD Biosciences
BUV805 Streptavidin
564923
Browse Similar Products: Streptavidin Flow Cytometry Antibodies
B cells have been shown to play a major role in pathogenesis of autoimmune diseases through presentation of antigen to T cells, production of autoantibodies and inflammatory cytokines that modulate immune responses. On the other hand, B cells act as a regulator through production of regulatory cytokine IL-10, inhibiting pathogenic T cells responses. In this part of the project we wanted to characterize a subset of B cell that express CD1d. It is a small population so using CD1d Biot SA to have a good signal was tried. In parallel, we tried to expand the panel using a SA labeled with a new fluorochrome.
Flow Cytometry
C57BL/6 spleen
CD1d biotin, 20 min at 4C
Fc block (2.4G2 clone)
SA BUV805
NA
Flow Cytometer with UV laser
We titrated the SA BUV805 and found that the SA can be use at 1:450.
N/A
It works
Is expensive
SA BUV805 can be used but it is not ideal.