BioLegend
Pacific Blue anti-human HLA-A,B,C Antibody
311417
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To measure HLA class I expression on monocytes, PBMC were isolated from peripheral blood of a healthy donor. PBMC were stained with viability dye, anti-human CD14, and anti-human HLA-A,B,C conjugated to Pacific Blue (clone: W6/32; manufacturer: BioLegend)
Flow Cytometry
PBMC from healthy human donor
Dilution 1:200; time 15 min; temp RT
Included in in-house staining buffer (PBS + 0.2% BSA)
N/A
Directly labelled
Great resolution of HLA class I expression on monocytes after gating on single viable CD14+ cells with monocyte characteristics in the side and forward scatter.
Great resolution at low titer
Potentially also detects HLA-E or HLA-G (https://www.ncbi.nlm.nih.gov/pubmed/25982269)
Recommended for global analysis of HLA class I expression