Phospho-Akt (Ser473) Antibody for Western Blot

February 01, 2019

University Health Network
Toronto General Hospital Research Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb

Catalog Number:

4060

Insulin receptor signaling leads to the phosphorylation of Akt at 2 residues- ser473 and thr308, which can be detected by western blotting.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Murine T Cell lysates

Primary Incubation

1/1000 4*C overnight in 5% BSA

Blocking Agent

5% BSA in TBS

Secondary Incubation

1/1000 anti rabbit HRP, 1 hour room temp

Tertiary Incubation

Not required

Detection

Chemiluminescence

Results Summary

Clean bands at expected molecular weight

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Reliable

The Bad

N/A

The Bottom Line

Works well for detecting pAkt at ser473

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