CD11b Antibody Great for Fast IHC

February 07, 2019

Pharmacology
Duke University
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor 488 anti-mouse/human CD11b Antibody

Catalog Number:

101219

This tandem antibody is great for fast CD11b staining of macrophages in tissue. Simply apply for 30-60 minutes at RT and then wash 3X in PBS. In summary, This stain works great with minimal off target or background. Cells are bright and clear in the sample with minimal bleaching effect allowing for continuous imaging for longer periods of time.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Dorsal Root Ganglia

Primary Incubation

PFA fixed tissue was washed 3X in PBS and blocked for 2 hours at RT with normal donkey serum. Tissue was then incubated 1:200 with AX488 anti -Cd11b for 30-60 minutes at RT. Washed 3X in PBS then confocal imaged.

Blocking Agent

Normal Donkey Serum

Secondary Incubation

None

Tertiary Incubation

None

Detection

Confocal Blue Laser

Results Summary

This stain works great with minimal off target or background. Cells are bright and clear in the sample with minimal bleaching effect allowing for continuous imaging for longer periods of time.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Quick and easy

The Bad

None noted

The Bottom Line

This is a quick and easy specific stain for macrophages in tissue.

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