Calcein Blue for Checking Single Cell Viability

Boston University
Center for Regenerative Medicine
Postdoctoral Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

ThermoFisher Scientific

Product Name:

eBioscience™ Calcein Blue AM Viability Dye

Catalog Number:

65-0855-39

The aim of this experiment was to digest whole lung tissue, collect single cells via fluorescence-activated cell sorting. The Calcein Blue AM was used to determine the viability of cells. The dye was recommended by colleagues.

Experimental Design and Results Summary

Application

Cell viability; fluorescence-activated cell sorter

Starting Material

Single cells

Protocol Overview

Stain cells right before taking samples to the sorter. 1 ul of dye per sample.

Tips

Cover reconstituted dye with foil or away from light.

Results Summary

Live and dead cells very easily distinguishable.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very easy to use. Reliable.

The Bad

None

The Bottom Line

Very reliable and cost-effective dye.

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