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Date Published
Phospho-Akt (Ser473) Antibody for Western Blot
(1)
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Cell Signaling Technology
February 01, 2019
Insulin receptor signaling leads to the phosphorylation of Akt at 2 residues- ser 473 and thr308, which can be detected by western blotting.
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Recombination Products When Cloning Repeat Elements
(1)
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New England Biolabs
February 01, 2019
I am trying to make DNA constructs that contain multiple repetitions of a target sequence of interest. I previously used another E. coli ...
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Mouse IgG2a Isotype Control Antibody
(1)
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February 01, 2019
FACS were performed with Mouse IgG2a isotype control antibody in parallel with CD24 to exclude false positive cells. In summary, viable CD24...
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Glutathione Measurement in MV4-11 Cells
(1)
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Enzo Life Sciences, Inc.
February 01, 2019
Kit protocol was followed to detect GSH levels in cells. Overall generated nice standard curve. In this experiment, samples were diluted 1:...
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PE Anti-Mouse CD120b (TNF R Type II/p75) Antibody
(1)
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BioLegend
February 01, 2019
This antibody was used for study of the cytokine receptor expression in splenic neutrophils. The antibody specifically labels CD120b but ...
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BAY-u 3405 Inhibits 15d-PGJ2-Induced p38 Activation
(1)
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Tocris Bioscience, a Bio-Techne Brand
February 01, 2019
HL-1 cardiomyocytes were pre-incubated with BAY-u 3405 (2 µM, 30 min) followed by treatment with 15d-PGJ2 (15 µM, 30 min) to ...
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Bright CD22 Expression Using ASR Reagent
(1)
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BD Biosciences
February 01, 2019
Identifying CD22 on B cells is an important part of our research. It inhibits B cell receptor signaling via alpha 2,6 sialic acid ligand ...
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Easy and Rapid Golgi Stain Kit
(1)
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February 01, 2019
Golgi impregnation made easy using this kit where all solutions are provided. The kit has been used to stain brain sections from P15–80 aged...
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Staining Mouse Splenocytes with Rat IgG2a BV421 Isotype Control
(1)
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BioLegend
February 01, 2019
This antibody was used as an isotype control to account for non-specific background staining in mouse splenocytes. It was chosen because it ...
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EGTA Inhibits 15d-PGJ2 Induced TNFa Production by Cardiomyocytes
(1)
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Tocris Bioscience, a Bio-Techne Brand
January 31, 2019
HL-1 cells were incubated with EGTA (5 mM) for 30 min prior to 15d-PGJ2 (15 µM) treatment for 4 hr to follow TNFa concentrations in the...
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Great Pipets
(1)
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January 31, 2019
These are lovely pipets. They look crisp and clean. They work well and have a comfortable ergonomic design and an easy-to-use locking ...
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F4/80 Antibody for Immunofluorescence
(1)
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Thermo Fisher Scientific
January 31, 2019
We are interested in the role of kidney resident macrophages in kidney inflammation. We wanted to investigate the location of kidney ...
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Use of EZ-10 Spin Column Plasmid DNA Miniprep Kits
(1)
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Bio Basic Inc.
January 31, 2019
This kit is convenient to use. We use it to do all the DNA purification. including miniprep. gel extraction. pcr purification.
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High Fidelity DNA Polymerase Compared to the Conventional Taq Polymerase
(1)
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January 31, 2019
This reagent is commonly used for PCR to amplify our target gene of interest and used for subsequently cloning into our vector plasmid. It ...
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Monoclonal FOXP3-APC Anti-Mouse Antibody
(1)
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Thermo Fisher Scientific
January 31, 2019
Regulatory T cells are a specialized subset of CD4+ T cells involved in suppression of immune responses and identified by the expression of ...
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Fura-2 AM Dye Detects Ca2+ Release from ER Stores in EA.hy926 Cells
(1)
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Thermo Fisher Scientific
January 31, 2019
EA.hy926 cells were loaded with cytosolic Ca2+ sensitive dye, Fura-2AM in normal tyrode solution for 40 min and excited alternately at 340 ...
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PE Mouse Anti-Human CD24 Antibody
(1)
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January 31, 2019
CD24+CD133+, CD24+CD133-, and CD24-CD133- cells isolated by flow cytometry on a FACS Aria II Cell Sorter (BD Biosciences) from human HCC ...
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Staining Mouse Splenocytes with Armenian Hamster IgG PerCP/Cy5.5 Isotype Control
(1)
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BioLegend
January 31, 2019
This antibody was used as an isotype control to account for non-specific background staining in mouse splenocytes. It was chosen because it ...
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PE Anti-Mouse CD120a (TNFaR Type I/p55) Antibody
(1)
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BioLegend
January 31, 2019
We used this antibody for identification of cytokine receptor expression in splenic neutrophils. The antibody specifically labels CD120a but...
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Brilliant Violet 510 Anti-Mouse CD25 Antibody
(1)
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BioLegend
January 30, 2019
I am studying molecular anomalies in signaling pathways leading to cancers and the development of anti-cancer immune therapies. This ...
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EDTA Inhibits 15d-PGJ2 Induced TNFa Production by Cardiomyocytes
(1)
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Tocris Bioscience, a Bio-Techne Brand
January 30, 2019
HL-1 cells were incubated with EDTA (5 mM) for 30 min prior to 15d-PGJ2 (15 µM) treatment for 4 hr to follow TNFa concentrations in the...
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CellROX™ Deep Red Reagent in EOL-1 Cells Assay Optimization
(1)
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Thermo Fisher Scientific
January 30, 2019
CellROX reagent was used in a flow cytometry assay with EOL-1 cells. Cells were treated with either NAC (negative control), or TBHP (...
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Excellent Dish for Confocal Imaging with Plastic TC Surface
(1)
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ibidi, LLC
January 30, 2019
This dish was used to attach recombinant protein to the surface and then perform inverted confocal microscopy. Worked perfectly to replicate...
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Staining Cultured MSC with Mouse IgG2b PE Isotype Control
(1)
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Abcam, BioLegend
January 30, 2019
This antibody was used as an isotype control to account for non-specific background staining in cultured MSC. It was chosen because it was ...
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Easy-to-Use SYBR Reagent for qPCR
(1)
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Bioline
January 30, 2019
The reagent is used for qPCR analysis to study the changes in the mRNA expression levels in the cell upon different treatments. The reagent ...
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Works but Requires a Lot of Unnecessary Effort
(1)
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Corning Life Sciences
January 30, 2019
If you need to spin things down in conventional tubes only, I guess this could be for you, though it is supremely lacking. Can't fit spin ...
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Doxycycline Hyclate for Cell Treatment
(1)
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MilliporeSigma
January 30, 2019
Development of stable doxycycline (Dox)-inducible PKD1 expression cell lines The doxycycline-inducible PKD1 expression cell lines were ...
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Easy-to-Use Glucose Analog for the Measurement of Glucose Uptake
(1)
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Cayman Chemical
January 30, 2019
Glucose is an essential fuel for multiple cell types including activated immune cells. The Cayman glucose uptake cell-based assay kit uses a...
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Amazing Teaching-Lab Assistant
(1)
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January 30, 2019
I work in an academic teaching lab where we prepare many aliquots of many solutions for many lab courses. We have had the controller for ...
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A Great Incubator Shaker
(1)
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Amerex Instruments, Inc.
January 29, 2019
I first used Gyromax 747R during my postdoc. There were several incubators in the lab but 747R was the most popular one, always booked for ...
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Phospho-Akt (Thr308) Antibody for Western Blot
(1)
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Cell Signaling Technology
January 29, 2019
Insulin receptor signaling leads to the phosphorylation of Akt at 2 residues- ser 473 and thr308, which can be detected by western blotting.
Read Review
Anti-Mouse PE IL6Ra Antibody
(1)
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BioLegend
January 29, 2019
We used this antibody for identification of IL-6Ra expression in Neutrophil subsets. The antibody specifically labels IL6Ra but is not very ...
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Staining Mouse Splenocytes with Anti-CD4 FITC
(1)
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BioLegend
January 29, 2019
This antibody was used to stain CD4+ cells in mouse splenocytes. It was chosen because it is a commonly used clone for CD4, FITC has the ...
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PE Anti-Mouse CD28 Antibody
(1)
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BioLegend
January 29, 2019
I am studying molecular anomalies in signaling pathways leading to cancers and the development of anti-cancer immune therapies. This ...
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Best Antibody for Human Antigen R Detection
(1)
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Santa Cruz Biotechnology, Inc.
January 29, 2019
HuR is a stabilizing RNA-binding protein that is encoded by the ELAV-like RNA-binding protein 1. It contains 3 RRMs (RNA recognition motifs)...
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Good TCR Antibody
(1)
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Thermo Fisher Scientific
January 29, 2019
Our lab studies the function of T cells in an influenza model in mice, and to do so we use various genetically modified mice. For some of ...
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Tempol Blunts 15d-PGJ2-Induced ROS Levels in HL-1 Cardiomyocytes
(1)
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Tocris Bioscience, a Bio-Techne Brand
January 29, 2019
HL-1 cardiomyocytes were incubated with Tempol (1 mM) for 30 min prior to 15d-PGJ2 treatments (15 μM) for 1 hr to follow ROS generation...
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SAHA Inhibitor
(1)
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Cayman Chemical
January 29, 2019
UPCI15B and UMSCC-1 cells were treated with SAHA and 5-aza-dC alone or in combination for 48 h. Cells were harvested for mRNA extraction and...
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A Must-Buy If You Have a Jenway 67 Series Spectrophotometer
(1)
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January 29, 2019
This replaces the 1-cell insert in the 67 series, and is a must-have if you take several measurements at once.
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Very Good Mouse TCR Vß5.1, 5.2 Antibody
(1)
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BioLegend
January 28, 2019
Our lab studies the function of T cells in an influenza model in mice, and to do so we use various genetically modified mice. In one of our ...
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15d-PGJ2 Promotes MAPK Activation in Cardiomyocytes
(1)
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Tocris Bioscience, a Bio-Techne Brand
January 28, 2019
Treatment of HL-1 cells (15 min) with increasing 15d-PGJ2 concentrations induced phosphorylation of p38 and p42/44 MAPK (shown in Image). A ...
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Very Easy and Efficient Taq Mastermix
(1)
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Promega
January 28, 2019
I needed a cheap and efficient PCR mix since I have to do a lot of PCR from bacteria colonies to check presence/absence of my target insert ...
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Rabbit His-Tag Antibody
(1)
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Thermo Fisher Scientific
January 28, 2019
Our main goal is to purify an N-terminus-histidine tagged protein and perform a binding assay to check its binding with a ubiquitous ...
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Staining Mouse Splenocytes with Anti-CD8 BV421
(1)
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BioLegend
January 28, 2019
This antibody was used to stain CD8+ cells in mouse splenocytes. It was chosen because it is a commonly used clone for CD8, BV421 is bright ...
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Detecting Lipid Peroxidation in MV4-11 Cells with Image-iT™ Lipid Peroxidation Kit
(1)
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Thermo Fisher Scientific
January 28, 2019
Following kit protocol to assess lipid peroxidation in MV4-11 cells treated with controls that came with kit (alpha-tocophenol (AT) and ...
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5-aza-dC (5-aza-2'-deoxycytidine) Inhibitor
(1)
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MilliporeSigma
January 28, 2019
UPCI15B and UMSCC-1 cells were treated with SAHA and 5-aza-dC alone or in combination for 48 h. Cells were harvested for mRNA extraction and...
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Great CD10 Antibody
(1)
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BD Biosciences
January 28, 2019
CD10 is a useful marker in the classification of pre B ALL. It is positive in 89% of cases. Our work on the identification of novel targets ...
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Great Kit for Fast and Easy Plasmid DNA Purification
(1)
Write a Review
January 28, 2019
We used to use the Qiagen Plasmid Maxi Kit for plasmid DNA purification. However, this kit was very laborious and took a long time with ...
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FB Essence: A Cost-Effective, Quality Alternative to FBS
(1)
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VWR International
January 28, 2019
We have been using FB Essence from VWR (VWR Life Science Seradigm FB Essence) since late 2016 and found it to be a very viable alternative ...
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The Antibody Stains PD-1+ Cells Well
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BioLegend
January 25, 2019
We wanted to study T cells in the diseased and healthy state, so we used the antibody to stain PD-1+ T cells in the lymph nodes. The ...
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