Used To Detect Biotinylated Proteins On Cell Surface By Flow Cytometry

Princeton
Molecular Biology
Graduate Student

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Company:

BD pharmigen

Product Name:

APC-streptavidin

Catalog Number:

554067

Extremely specific and low variability for detection of biotin labeling of cell surface proteins.

Experimental Design and Results Summary

Application

Flow cytometry

Starting Material

Oxidized breast cancer cell

Protocol Overview

Cell surface was chemically modified via biotinylation and then the extent of biotinylation was quantified using this reagent at 1:200.

Tips

Pretty fool-proof

Results Summary

Results showed a single peak with a very narrow base depicting much lower variability than normal antibody labels.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Great binding and very specific signal

The Bad

none

The Bottom Line

Excellent and amplified alternative to antibody detection.

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