Thermo Fisher Scientific
Polyoma Virus Medium T Monoclonal Antibody
Ma1-46061
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Browse Similar Products: PyMT Immunofluorescence Antibodies
We study mammary gland tumorigenesis in the PyMT mouse model. We wanted to detect metastatic PyMT cells. As a positive control, we stained mammary gland with excellent results.
Immunofluorescence
PLP fixed mouse (C57BL/6) PyMT mammary gland cryosections
1 in 200 for 3 hr at room temperature
1% BSA, 1% FCS, 0.3% Triton X, 2% mouse serum, 2% rat serum
Goat anti-rabbit Alexa Fluor 647 (Thermofisher, A-21245) 1:500
N/A
Images were acquired using an inverted Zeiss LSM880 confocal microscope with a 20x 0.8NA air objective
Excellent staining of PyMT (green). DAPI is in blue.
Excellent staining
None
Excellent & reproducible staining.