IL-6 Human ELISA - Great

Duke University
Pharmacology
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fischer Scientific

Product Name:

IL-6 Human Uncoated ELISA

Catalog Number:

88-7066-22

THP-1 cells were treated with LPS for 24 hours and treated with a TAK1 inhibitor. The ELISA kit was able to show IL-6 levels in cell culture. This was very sensitive and needed to be optimized for appropriate dilutions.

Experimental Design and Results Summary

Application

ELISA

Starting Material

Human THP-1 cells

Protocol Overview

THP-1 cells cultured with LPS were treated with TAK1 inhibitors and showed reduction in IL-6 secretion in a dose-dependent manner.

Tips

Find the right dilutions.

Results Summary

Works great once the proper dilution is determined.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Works well and sensitive

The Bad

None noted

The Bottom Line

Works great for IL-6 Elisa of cell culture or serum samples.

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