Non-Specific Antibody that Does Not Clearly Detect Fut7

February 21, 2019

BlueskyReddit
Molecular Biology
Princeton
Graduate Student

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Company:

Proteintech

Product Name:

FUT7 Antibody

Catalog Number:

18197-1-AP

Clone Number:

This antibody does not work to detect human Fut7 by western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

MDA-MB-231 cells expressing hFut7

Primary Incubation

1:1000

Blocking Agent

5% milk

Secondary Incubation

HRP-anti-rabbit

Tertiary Incubation

None

Detection

Chemiluminescent

Results Summary

Results showed no specific bands at the expected size in the overexpressed line, while many other bands were strongly detected after a short incubation.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

None

The Bad

It doesn't work

The Bottom Line

Find a different Fut7 antibody

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