Works After Optimization

February 19, 2019

Pharmacology
Duke University
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

BioLegend

Product Name:

CD 39 PE

Catalog Number:

143803

This antibody works after optimization. It needs to be used in unfixed cells. Following PFA fixation it does not work.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse blood monocytes

Primary Incubation

1:50

Blocking Agent

CD16/32

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR II

Results Summary

This antibody only works in unfixed cells. It also isn't the brightest but can work best when titrated for appropriate fluorescence.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works in fresh immune cells

The Bad

Doesn't work post fixation

The Bottom Line

This works after some small optimization.

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