Ultrapure Water for Highly Sensitive Experiments

Harvard Medical School
Cell Biology
Postdoctoral Fellow

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Company:

Invitrogen Life Technologies

Product Name:

UltraPure Distilled Water

Catalog Number:

10977015

While for most experiments we use Milli-Q water or just regular distilled water, some sensitive experiments require much more pure water to generate reproducible results. We have been using this brand of ultra pure water for our mass spec sample preparation, enzymatic assays, and buffers for embryonic stem cell culture.

Experimental Design and Results Summary

Application

Mass spectrometry based proteomics

Starting Material

N/A

Protocol Overview

N/A

Tips

Be careful not to contaminate the water

Results Summary

For sensitive experiments, using this commercial ultra pure water can enhance the reproducibility of results. While most of the time, Milli-Q water is good enough, we have experienced that the buffer made from Milli-Q water makes ESCs less viable, and by changing to this brand of ultra pure water, the cells recovered.

DOI or PMID #

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Additional Notes

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Summary

The Good

Reliable source of ultra pure water for sensitive experiments

The Bad

A little more expensive

The Bottom Line

It is well worth it to use this water for sensitive and important experiments.

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