Fast, Reproducible Dissociation of Adult Mouse Brain

Georgetown University Medical Center
Pharmacology and Physiology
PhD Candidate

Overall

Performance

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

gentleMACS Octo Dissociator with Heaters

Catalog Number:

130-096-427

The gentleMACS Octo with heaters is the only dissociator that Miltenyi rates for use with their adult brain dissociation kit. We had no problem at all setting up and running the programs, and were able to create seemingly viable single-cell suspensions for our downstream applications.

Experimental Design and Results Summary

Application

Adult Mouse Brain Dissociation

Protocol Overview

We strictly followed the manufacturer's instructions, using the 30 mins at 37°C program to dissociate 1 mouse brain per tube.

Tips

Do not let the tissue slurry overdigest - take it out as soon as the dissociation program is finished.

Features Summary

Performs mechanical and enzymatic dissociation by using a grinding motor and tube, and heaters.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Easy to use, can run 8 samples/programs independently

The Bad

Expensive solution, but worth it for the reproducibility

The Bottom Line

This is a great tool for dissociating a variety of tissues into single-cell suspensions or even making them into lysates (with different, tighter grinder tubes). The design allows both mechanical and enzymatic dissociation to occur simultaneously and reproducibly, unlike manual trituration/homogenization and then incubation in a water bath. Being able to run many samples independently at once is also very helpful for batch processing without having to wait for the usual bottleneck of tissue collection.

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