Detection of Epithelial Cells in Whole Lung Tissue

February 26, 2019

Center for Regenerative Medicine
Boston University
Postdoctoral Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

Ep-CAM Antibody (EBA-1) Alexa Fluor® 647

Catalog Number:

sc-66020 AF647

The aim of this experiment was to detect epithelial cells in whole lung tissue of rats by FACS. The product was identified through a search on the Santa Cruz website.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Whole lung tissue

Primary Incubation

2 µL

Blocking Agent

Rat Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

FACS Aria II

Results Summary

Epithelial cells were detected.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use

The Bad

Expensive for a conjugated antibody

The Bottom Line

Rat antibodies are difficult to source, so finding one that works is a plus point.

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