Cytokine Detection

Cytokine detection is important when studying cellular communication, immune response, inflammation and hematopoiesis. Cytokines, which are small signaling proteins, have historicaly have been grouped based on criteria such as protein structure and function. These subgroups include interleukins (secreted by leukocytes), chemokines (chemotaxis-inducing), interferons (antiviral activity) and tumor necrosis factors (containing the TNF structural domain). Based on their role in controlling inflammation, they are also subdivided as either proinflammatory and anti-inflammatory cytokines. When investigating cytokines, an immunodetection approach is common in which cytokine-specific antibodies are used in staining, ELISA, or other types of immunoassay. Intracellular cytokine detection can also be accomplished by flow cytometry after labeling the interior of cells antibodies with fluorescent reporters. Important features to look for in cytokine assay kits are sensitivity, broad dynamic range, and quality of the antibody pairs’ affinity and specificity.

Recommended Reading:

Cytokines, Their Detection, and Their Roles in Disease

Scientists need reliable methods for detection cytokines, and measuring their levels in different conditions. This article looks at tools for common methods of cytokine detection, and how cytokine detection intersects with a wide variety of disease studies.
Seeking Out Cytokines

Beginning in the 1940s, scientists have studied the many disease-related roles of cytokines. Despite more than 70 years of research, these small messengers remain a difficult target to track, but well worth the effort, especially in medical applications.